Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | stimulates | Oenococcus oeni | |
DTT | stimulates | Oenococcus oeni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Oenococcus oeni | |
Co2+ | - |
Oenococcus oeni | |
Cu2+ | - |
Oenococcus oeni | |
EDTA | - |
Oenococcus oeni | |
Fe2+ | - |
Oenococcus oeni | |
Hg2+ | - |
Oenococcus oeni | |
Zn2+ | - |
Oenococcus oeni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
2-oxoglutarate | pH 5.3, 30°C | Oenococcus oeni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
1 * 65000, SDS-PAGE | Oenococcus oeni |
70000 | - |
PAGE | Oenococcus oeni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oenococcus oeni | - |
B 211 | - |
Oenococcus oeni B 211 | - |
B 211 | - |
Purification (Comment) | Organism |
---|---|
- |
Oenococcus oeni |
Storage Stability | Organism |
---|---|
4°C or -20°C, 24 h, 95% or 25% loss of activity, respectively | Oenococcus oeni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate | - |
Oenococcus oeni | succinate semialdehyde + CO2 | - |
? | |
2-oxoglutarate | - |
Oenococcus oeni B 211 | succinate semialdehyde + CO2 | - |
? | |
oxaloacetate | 28% of the activity with 2-oxoglutarate | Oenococcus oeni | ? | - |
? | |
oxaloacetate | 28% of the activity with 2-oxoglutarate | Oenococcus oeni B 211 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, SDS-PAGE | Oenococcus oeni |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 5.3, 30 min, 10% loss of activity | Oenococcus oeni |
70 | - |
pH 5.3, 5 min, 90% loss of activity | Oenococcus oeni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
- |
Oenococcus oeni |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | active in the range | Oenococcus oeni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | required | Oenococcus oeni | |
thiamine diphosphate | 0.03 mM | Oenococcus oeni |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oenococcus oeni | isoelectric focusing | - |
4.2 |