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Literature summary for 4.1.1.71 extracted from

  • Kapol, R.; Radler, F.
    Purification and characterization of 2-oxoglutarate decarboxylase of Leuconostoc mesenteroides (1990), J. Gen. Microbiol., 136, 1497-1499.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates Oenococcus oeni
DTT stimulates Oenococcus oeni

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Oenococcus oeni
Co2+
-
Oenococcus oeni
Cu2+
-
Oenococcus oeni
EDTA
-
Oenococcus oeni
Fe2+
-
Oenococcus oeni
Hg2+
-
Oenococcus oeni
Zn2+
-
Oenococcus oeni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
2-oxoglutarate pH 5.3, 30°C Oenococcus oeni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
1 * 65000, SDS-PAGE Oenococcus oeni
70000
-
PAGE Oenococcus oeni

Organism

Organism UniProt Comment Textmining
Oenococcus oeni
-
B 211
-
Oenococcus oeni B 211
-
B 211
-

Purification (Commentary)

Purification (Comment) Organism
-
Oenococcus oeni

Storage Stability

Storage Stability Organism
4°C or -20°C, 24 h, 95% or 25% loss of activity, respectively Oenococcus oeni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate
-
Oenococcus oeni succinate semialdehyde + CO2
-
?
2-oxoglutarate
-
Oenococcus oeni B 211 succinate semialdehyde + CO2
-
?
oxaloacetate 28% of the activity with 2-oxoglutarate Oenococcus oeni ?
-
?
oxaloacetate 28% of the activity with 2-oxoglutarate Oenococcus oeni B 211 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Oenococcus oeni

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
pH 5.3, 30 min, 10% loss of activity Oenococcus oeni
70
-
pH 5.3, 5 min, 90% loss of activity Oenococcus oeni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
-
Oenococcus oeni

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 active in the range Oenococcus oeni

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate required Oenococcus oeni
thiamine diphosphate 0.03 mM Oenococcus oeni

pI Value

Organism Comment pI Value Maximum pI Value
Oenococcus oeni isoelectric focusing
-
4.2