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Literature summary for 4.1.1.7 extracted from
- Covalent immobilization of benzoylformate decarboxylase from Pseudomonas putida on magnetic epoxy support and its carboligation reactivity (2014), J. Mol. Catal. B, 102, 188-194.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His-tagged enzyme in Escherichia coli strain SG13009 | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | covalent immobilization and stabilization of benzoylformate decarboxylase from Pseudomonas putida on magnetic nanoparticles by a three-step immobilization/stabilization procedure at pH 8.0, 20°C, method development and evaluation, overview. The maximum enzyme loading is found to be 5.2 mg/g support in 3.5 mg/ml initial enzyme concentration at ionic strength of 1.25 M MgSO4. The covalently-bound enzyme is characterized in terms of its activity and stability for the formation of (S)-2-hydroxypropiophenone. The immobilized biocatalyst retains 95% of its original activity after five reaction cycles | Pseudomonas putida |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Pseudomonas putida |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | synthesis of (S)-2-hydroxy-1-phenylpropanone by the immobilized recombinant enzyme is effective in cross acyloin reaction of benzaldehyde with acetaldehyde. The immobilized and free enzymes shows similar substrate specificity and activity | Pseudomonas putida | ? | - |
? | |
| phenylglyoxylate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BFD | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at, substrate phenylglyoxylate | Pseudomonas putida |
| 8.5 | - |
assay at, cross acyloin reaction of benzaldehyde with acetaldehyde | Pseudomonas putida |
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