Application | Comment | Organism |
---|---|---|
synthesis | the enzyme recombinantly expressed in Escherichia coli is useful for production of ethyl vanillin, i.e. 3-ethoxy-4-hydroxybenzaldehyde, which is a good substitute for vanillin in foods and perfumes since it is 3.5times stronger in flavor and keeps better condition in storage and transport | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
gene mdlC, recombinant expression in Escherichia coli strain BL21(DE3), suncloning in Escherichia coli strain DH5alpha | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | biotransformation using recycled resting Escherichia coli cells expressing the enzyme, the product yield is above 42% after four repeated cycles | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-ethoxy-4-hydroxybenzoylformate | Pseudomonas putida | - |
ethyl vanillin + CO2 | - |
? | |
3-ethoxy-4-hydroxybenzoylformate | Pseudomonas putida ATCC 12633 | - |
ethyl vanillin + CO2 | - |
? | |
additional information | Pseudomonas putida | when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview | ? | - |
? | |
additional information | Pseudomonas putida ATCC 12633 | when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview | ? | - |
? | |
phenylglyoxylate | Pseudomonas putida | - |
benzaldehyde + CO2 | - |
? | |
phenylglyoxylate | Pseudomonas putida ATCC 12633 | - |
benzaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P20906 | gene mdlC | - |
Pseudomonas putida ATCC 12633 | P20906 | gene mdlC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-ethoxy-4-hydroxybenzoylformate | - |
Pseudomonas putida | ethyl vanillin + CO2 | - |
? | |
3-ethoxy-4-hydroxybenzoylformate | - |
Pseudomonas putida ATCC 12633 | ethyl vanillin + CO2 | - |
? | |
additional information | when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview | Pseudomonas putida | ? | - |
? | |
additional information | when 3-ethoxy-4-hydroxybenzoylformate is provided at with Pseudomonas putida, it is completely transformed to ethyl vanillic acid after 2 days, but only trace amounts of ethyl vanillin are detected. Similar results are obtained when ethyl vanillin replaces 3-ethoxy-4-hydroxybenzoylformate as substrate, thus aldehyde dehydrogenation activity in Pseudomonas putida is greater than its synthesis activity. The recombinant Escherichia coli strain expressing the enzyme from Pseudomonas putida converts (S)-3-ethoxy-4-hydroxymandelic acid into ethyl vanillin, but does not degrade ethyl vanillin, overview | Pseudomonas putida ATCC 12633 | ? | - |
? | |
phenylglyoxylate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? | |
phenylglyoxylate | - |
Pseudomonas putida ATCC 12633 | benzaldehyde + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas putida |