Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, crystal structure of Q52A mutant and wild-type enzyme in complex with pyridoxamine 5'-phosphate | Burkholderia cepacia |
Protein Variants | Comment | Organism |
---|---|---|
Q52A | 85fold decrease in turnover number for decarboxylation half-reaction with 2-aminoisobutyrate and pyruvate, greatly reduced rate of transamination half-reaction and impaired pyridoxamine phosphate binding | Burkholderia cepacia |
Q52E | 10000fold decrease in turnover number for decarboxylation half-reaction with 2-aminoisobutyrate and pyruvate, transamination rate is nearly identical to wild-type value | Burkholderia cepacia |
Q52I | 100000fold decrease in turnover number for decarboxylation half-reaction with 2-aminoisobutyrate and pyruvate, greatly reduced rate of transamination half-reaction and impaired pyridoxamine phosphate binding | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
pyruvate | pH 7.8, mutant enzyme Q52A | Burkholderia cepacia | |
30 | - |
2-aminoisobutanoate | pH 7.8, mutant enzyme Q52A | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | P16932 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Aminoisobutanoate + pyruvate | - |
Burkholderia cepacia | L-Ala + acetone + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
pyruvate | pH 7.8, mutant enzyme Q52A | Burkholderia cepacia | |
0.12 | - |
2-aminoisobutanoate | pH 7.8, mutant enzyme Q52A | Burkholderia cepacia |