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Literature summary for 4.1.1.63 extracted from

  • Yoshida, T.; Inami, Y.; Matsui, T.; Nagasawa, T.
    Regioselective carboxylation of catechol by 3,4-dihydroxybenzoate decarboxylase of Enterobacter cloacae P (2010), Biotechnol. Lett., 32, 701-705.
    View publication on PubMed
Search for more literature for 4.1.1.63

General Stability

General Stability Organism
addition of 5 mM dithiothreitol and 20 mM Na2S2O3 to a cell-free extract stabilizes the enzyme Enterobacter cloacae

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
-
-
Enterobacter cloacae P241
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterobacter cloacae

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
4°C, cell-free extract in the absence of sulfhydryl-protecting reagents, 2 h, 50% loss of activity Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-Dihydroxybenzoate 28% conversion after 14 h at 30°C Enterobacter cloacae Catechol + CO2
-
 ?
3,4-Dihydroxybenzoate 28% conversion after 14 h at 30°C Enterobacter cloacae P241 Catechol + CO2
-
 ?
catechol + KHCO3 28% conversion after 14 h at 30°C Enterobacter cloacae 3,4-dihydroxybenzoate
-
 ?
catechol + KHCO3 28% conversion after 14 h at 30°C Enterobacter cloacae P241 3,4-dihydroxybenzoate
-
 ?

Synonyms

Synonyms Comment Organism
3,4-dihydroxybenzoate decarboxylase
-
 Enterobacter cloacae

Expression

Organism Comment Expression
Enterobacter cloacae 3,4-dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 is induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium up