Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | 1 mM pyridoxal 5'-phosphate or thiamine diphosphate do not enhance activity | Enterobacter cloacae |
Crystallization (Comment) | Organism |
---|---|
- |
Enterobacter cloacae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | 2.5 mM, 91% loss of activity | Enterobacter cloacae | |
CuSO4 | 2.5 mM, 92% loss of activity | Enterobacter cloacae | |
EDTA | 2.5 mM, 22% inhibition | Enterobacter cloacae | |
HgCl2 | 2.5 mM, 78% loss of activity | Enterobacter cloacae | |
additional information | not significantly affected by phenylhydrazine or hydroxylamine | Enterobacter cloacae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.596 | - |
4-hydroxybenzoate | in 100 mM potassium phosphate buffer (pH 7.0) | Enterobacter cloacae | |
6.8 | - |
3,4-dihydroxybenzoate | in 100 mM potassium phosphate buffer (pH 7.0) | Enterobacter cloacae | |
14.8 | - |
phenol | 20 mM phenol, 3 MKHCO3, 10 mM dithiothreitol in 100 mM potassium phosphate buffer (pH 7.0) | Enterobacter cloacae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 2.5 mM, 99% stimulation of activity | Enterobacter cloacae | |
CdCl2 | 2.5 mM, 51% stimulation of activity | Enterobacter cloacae | |
MgSO4 | 2.5 mM, 125% stimulation of activity | Enterobacter cloacae | |
MnCl2 | 2.5 mM, 112% stimulation of activity | Enterobacter cloacae | |
additional information | AgNO3, FeCl2, CoCl2, NiCl2, and FeSO4 do not affect activity | Enterobacter cloacae | |
ZnCl2 | 2.5 mM, 75% stimulation of activity | Enterobacter cloacae | |
ZnSO4 | 2.5 mM, 100% stimulation of activity | Enterobacter cloacae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
6 * 60000, X-ray crystallography | Enterobacter cloacae |
315800 | - |
calculated from amino acid sequence | Enterobacter cloacae |
372000 | - |
gel-filtration HPLC | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
- |
- |
Enterobacter cloacae P240 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sephacel column chromatography, phenyl-Sepharose CL-4B column chromatography, and butyl-Toyopearl 650M column chromatography | Enterobacter cloacae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:4-hydroxybenzoate-grown cell | - |
Enterobacter cloacae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.7 | - |
at 30°C, pH 7.0, crude cell extract | Enterobacter cloacae |
17.8 | - |
at 30°C, pH 7.0, after 1.5fold purification | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate | activity toward 3,4-dihydroxybenzoate at 5 mM is 2.8% of that toward 4-hydroxybenzoate | Enterobacter cloacae | benzene-1,2-diol + CO2 | - |
? | |
3,4-dihydroxybenzoate | activity toward 3,4-dihydroxybenzoate at 5 mM is 2.8% of that toward 4-hydroxybenzoate | Enterobacter cloacae P240 | benzene-1,2-diol + CO2 | - |
? | |
4-Hydroxybenzoate | - |
Enterobacter cloacae | Phenol + CO2 | - |
r | |
4-Hydroxybenzoate | - |
Enterobacter cloacae P240 | Phenol + CO2 | - |
r | |
additional information | benzoate, 2-hydroxybenzoate, 3-hydroxybenzoate, 2,6-dihydroxybenzoate, 2,4,6-trihydroxybenzoate, 3,4,5-trihydroxybenzoate, terephthalate, vanillate, 4-methoxybenzoate, 4-aminobenzoate, and 6-hydroxy-2-naphthoate are inert as substrates | Enterobacter cloacae | ? | - |
? | |
additional information | benzoate, 2-hydroxybenzoate, 3-hydroxybenzoate, 2,6-dihydroxybenzoate, 2,4,6-trihydroxybenzoate, 3,4,5-trihydroxybenzoate, terephthalate, vanillate, 4-methoxybenzoate, 4-aminobenzoate, and 6-hydroxy-2-naphthoate are inert as substrates | Enterobacter cloacae P240 | ? | - |
? | |
Phenol + CO2 | carboxylation in the presence of 3 M KHCO3 and 20 mM phenol with a molar conversion yield of 19% | Enterobacter cloacae | 4-Hydroxybenzoate | - |
? | |
Phenol + CO2 | carboxylation in the presence of 3 M KHCO3 and 20 mM phenol with a molar conversion yield of 19% | Enterobacter cloacae P240 | 4-Hydroxybenzoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 60000, X-ray crystallography | Enterobacter cloacae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in potassium phosphate buffer | Enterobacter cloacae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
when the enzyme is incubated at temperatures up to 20°C for 30 min, activity is barely decreased | Enterobacter cloacae |
30 | 60 | treatment at 30, 40, 50, and 60°C causes 32, 43, 96, and 100% losses of the initial activity, respectively | Enterobacter cloacae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
in potassium phosphate buffer | Enterobacter cloacae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Enterobacter cloacae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the activity of 4-hydroxybenzoate decarboxylase is independent of pyridoxal 5'-phosphate, thiamine diphosphate, and the pyruvoyl group | Enterobacter cloacae |