Application | Comment | Organism |
---|---|---|
synthesis | synthesis of pyrogallol from readily available tannic acid by using the combined action of gallate decarboxylase and tannase in a two-enzyme resting cell bioconversion | Pantoea agglomerans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2S2O8 | - |
Pantoea agglomerans | |
1,10-phenanthroline | - |
Pantoea agglomerans | |
2,2'-bipyridyl | - |
Pantoea agglomerans | |
Ag2SO4 | - |
Pantoea agglomerans | |
CuCl2 | - |
Pantoea agglomerans | |
Cuprizone | - |
Pantoea agglomerans | |
EDTA | - |
Pantoea agglomerans | |
FeCl2 | slight | Pantoea agglomerans | |
FeCl3 | slight | Pantoea agglomerans | |
H2O2 | - |
Pantoea agglomerans | |
HgCl2 | - |
Pantoea agglomerans | |
K2CrO4 | - |
Pantoea agglomerans | |
KCN | - |
Pantoea agglomerans | |
NaNO3 | - |
Pantoea agglomerans | |
p-chloromercuribenzoate | - |
Pantoea agglomerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.96 | - |
3,4,5-Trihydroxybenzoate | - |
Pantoea agglomerans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | required as cofactor, contains 0.8 mol of iron per mol of enzyme subunit | Pantoea agglomerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
6 * 57000, SDS-PAGE | Pantoea agglomerans |
320000 | - |
gel filtration | Pantoea agglomerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea agglomerans | - |
i.e. Enterobacter agglomerans, strain T71 | - |
Oxidation Stability | Organism |
---|---|
oxygen-sensitive enzyme | Pantoea agglomerans |
Purification (Comment) | Organism |
---|---|
- |
Pantoea agglomerans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
150 | - |
- |
Pantoea agglomerans |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate buffer, pH 6.0, containing glycerol and reducing agents, stable for 3 weeks | Pantoea agglomerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4,5-Trihydroxybenzoate | reverse reaction not catalyzed | Pantoea agglomerans | Pyrogallol + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 57000, SDS-PAGE | Pantoea agglomerans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pantoea agglomerans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable below | Pantoea agglomerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pantoea agglomerans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | stable in the range | Pantoea agglomerans |