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Literature summary for 4.1.1.50 extracted from
- Crenarchaeal arginine decarboxylase evolved from an S-adenosylmethionine decarboxylase enzyme (2008), J. Biol. Chem., 283, 25829-25838.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | modeling and activity of a chimeric arginine decarboxylase/S-adenosylmethionine decarboxylase proteins. A chimeric protein containing the beta subunit of arginine decarboxylase (SSO0536) and the alpha subunit of S-adenosylmethionine decarboxylase (SSO0585) has arginine decarboxylase activity and no S-adenosylmethionine decarboxylase activity, implicating residues responsible for substrate specificity in the beta subunit | Saccharolobus solfataricus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16602 | - |
2 * 16602, electrospray ionization mass spectrometry | Saccharolobus solfataricus |
| 16680 | - |
2 * 16680, calculated from sequence | Saccharolobus solfataricus |
| 33000 | - |
His10-tagged enzyme, gel filtration | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine | Saccharolobus solfataricus | - |
(5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2 | - |
? |  |
| S-adenosyl-L-methionine | Saccharolobus solfataricus P2 | - |
(5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q9UWY8 | - |
- |
| Saccharolobus solfataricus P2 | Q9UWY8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.11 | - |
pH 8.0, 70°C | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine | - |
Saccharolobus solfataricus | (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2 | - |
? | |
| S-adenosyl-L-methionine | the crenarchaeal S-adenosylmethionine decarboxylase has no arginine decarboxylase activity | Saccharolobus solfataricus | (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2 | - |
? | |
| S-adenosyl-L-methionine | - |
Saccharolobus solfataricus P2 | (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2 | - |
? | |
| S-adenosyl-L-methionine | the crenarchaeal S-adenosylmethionine decarboxylase has no arginine decarboxylase activity | Saccharolobus solfataricus P2 | (5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium salt + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 16602, electrospray ionization mass spectrometry | Saccharolobus solfataricus |
| dimer | 2 * 16680, calculated from sequence | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AdoMetDC | - |
Saccharolobus solfataricus |
| protein SSO0585 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharolobus solfataricus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
S-adenosyl-L-methionine | pH 8.0, 70°C | Saccharolobus solfataricus | |
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Release 2023.1 (January 2023)
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