Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the AdoMetDC activity in the bifunctional enzyme is not stimulated by putrescine | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star cells | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
C505S | the expression level and specific activity of the mutant is unchanged compared to the wild type enzyme. The mutant protein occurs mainly as a monomer | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CGP48664 | non-competitive inhibition, i.e. 4-amidinoindan-1-one-2'-amidinohydrazone | Plasmodium falciparum | |
MDL73811 | irreversible inhibitor, i.e. 5'-([(Z)-4-amino-2-butenyl]methylamino)-5'-deoxyadenosine | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
S-adenosyl-L-methionine | bifunctional AdoMetDC/ODC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
0.058 | - |
S-adenosyl-L-methionine | native wild type AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
0.25 | - |
S-adenosyl-L-methionine | recombinant AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
9000 | - |
1 * 70000 + 1 * 9000, recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
70000 | - |
1 * 70000 + 1 * 9000, recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Strep-Tactin affinity chromatography | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.088 | - |
native wild type AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C | Plasmodium falciparum |
0.14 | - |
recombinant AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C | Plasmodium falciparum |
Storage Stability | Organism |
---|---|
-20°C, native wild type enzyme, 2 weeks, 55% loss of activity | Plasmodium falciparum |
4°C and -20°C, recombinant enzyme, 2 weeks, no loss of activity | Plasmodium falciparum |
4°C, native wild type enzyme, 2 weeks, 16% loss of activity | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | - |
Plasmodium falciparum | (5-deoxy-5-adenosyl)(3-aminopropyl)-methylsulfonium salt + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 70000 + 1 * 9000, recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
AdoMetDC/ODC | bifunctional enzyme | Plasmodium falciparum |
S-adenosylmethionine decarboxy-lase/ornithine decarboxylase | bifunctional enzyme | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.088 | - |
S-adenosyl-L-methionine | recombinant AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00033 | - |
MDL73811 | recombinant AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
0.0016 | - |
MDL73811 | bifunctional AdoMetDC/ODC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
0.003 | - |
CGP48664 | native wild type AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C | Plasmodium falciparum | |
0.00407 | - |
CGP48664 | recombinant AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C | Plasmodium falciparum |