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Literature summary for 4.1.1.50 extracted from

  • Williams, M.; Sprenger, J.; Human, E.; Al-Karadaghi, S.; Persson, L.; Louw, A.I.; Birkholtz, L.M.
    Biochemical characterisation and novel classification of monofunctional S-adenosylmethionine decarboxylase of Plasmodium falciparum (2011), Mol. Biochem. Parasitol., 180, 17-26.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the AdoMetDC activity in the bifunctional enzyme is not stimulated by putrescine Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star cells Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
C505S the expression level and specific activity of the mutant is unchanged compared to the wild type enzyme. The mutant protein occurs mainly as a monomer Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
CGP48664 non-competitive inhibition, i.e. 4-amidinoindan-1-one-2'-amidinohydrazone Plasmodium falciparum
MDL73811 irreversible inhibitor, i.e. 5'-([(Z)-4-amino-2-butenyl]methylamino)-5'-deoxyadenosine Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
S-adenosyl-L-methionine bifunctional AdoMetDC/ODC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C Plasmodium falciparum
0.058
-
S-adenosyl-L-methionine native wild type AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C Plasmodium falciparum
0.25
-
S-adenosyl-L-methionine recombinant AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
9000
-
1 * 70000 + 1 * 9000, recombinant enzyme, SDS-PAGE Plasmodium falciparum
70000
-
1 * 70000 + 1 * 9000, recombinant enzyme, SDS-PAGE Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin affinity chromatography Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.088
-
native wild type AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C Plasmodium falciparum
0.14
-
recombinant AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C Plasmodium falciparum

Storage Stability

Storage Stability Organism
-20°C, native wild type enzyme, 2 weeks, 55% loss of activity Plasmodium falciparum
4°C and -20°C, recombinant enzyme, 2 weeks, no loss of activity Plasmodium falciparum
4°C, native wild type enzyme, 2 weeks, 16% loss of activity Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine
-
Plasmodium falciparum (5-deoxy-5-adenosyl)(3-aminopropyl)-methylsulfonium salt + CO2
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 70000 + 1 * 9000, recombinant enzyme, SDS-PAGE Plasmodium falciparum

Synonyms

Synonyms Comment Organism
AdoMetDC/ODC bifunctional enzyme Plasmodium falciparum
S-adenosylmethionine decarboxy-lase/ornithine decarboxylase bifunctional enzyme Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.088
-
S-adenosyl-L-methionine recombinant AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00033
-
MDL73811 recombinant AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C Plasmodium falciparum
0.0016
-
MDL73811 bifunctional AdoMetDC/ODC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C Plasmodium falciparum
0.003
-
CGP48664 native wild type AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C Plasmodium falciparum
0.00407
-
CGP48664 recombinant AdoMetDC enzyme, in 50 mM KH2PO4 pH 7.5, 1 mM EDTA, 1 mM dithiothreitol, at 37°C Plasmodium falciparum