Literature summary for 4.1.1.49 extracted from

Bazaes, S.; Toncio, M.; Laivenieks, M.; Zeikus, J.G.; Cardemil, E.
Comparative kinetic effects of Mn (II), Mg (II) and the ATP/ADP ratio on phosphoenolpyruvate carboxykinases from Anaerobiospirillum succiniciproducens and Saccharomyces cerevisiae (2007), Protein J., 26, 265-269.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	-	oxaloacetate	condition: + 2 mM ADP /Vmax: 0.32 micromol/min/mg	Saccharomyces cerevisiae
0.079	-	oxaloacetate	condition: no ADP /Vmax: 3.27 micromol/min/mg	Anaerobiospirillum succiniciproducens
0.36	-	CO2	condition: 0.1 mM Mn2+, 5 mM Mg2 /Vmax: 16 micromol/min/mg	Saccharomyces cerevisiae
0.43	-	oxaloacetate	condition: no ADP /Vmax: 1.51 micromol/min/mg	Saccharomyces cerevisiae
0.99	-	oxaloacetate	condition: + 2 mM ADP /Vmax: 3.36 micromol/min/mg	Anaerobiospirillum succiniciproducens
1.9	-	CO2	condition: 0.1 mM Mn2+, 5 mM Mg2 /Vmax: 37 micromol/min/mg	Anaerobiospirillum succiniciproducens
3.1	-	CO2	condition: 4 mM Mn2+ /Vmax: 60 micromol/min/mg	Saccharomyces cerevisiae
8.4	-	CO2	condition: 4 mM Mn2+ /Vmax: 47 micromol/min/mg	Anaerobiospirillum succiniciproducens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Saccharomyces cerevisiae
Mg2+	-	Anaerobiospirillum succiniciproducens
Mn2+	-	Saccharomyces cerevisiae
Mn2+	-	Anaerobiospirillum succiniciproducens

Organism

Organism	UniProt	Comment	Textmining
Anaerobiospirillum succiniciproducens	-	-	-
Saccharomyces cerevisiae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + oxaloacetate	-	Saccharomyces cerevisiae	ADP + phosphoenolpyruvate + CO2	-	r
ATP + oxaloacetate	for the Anaerobiospirillum succiniciproducens enzyme, at physiological concentrations of Mn2+ and Mg2+, the affinity for CO2 increases as the ATP/ADP ratio is increased in the assay medium. The results show that a high ATP/ADP ratio favors CO2 fixation by the PEP carboxykinase from Anaerobiospirillum succiniciproducens	Anaerobiospirillum succiniciproducens	ADP + phosphoenolpyruvate + CO2	-	r

Synonyms

Synonyms	Comment	Organism
PEP carboxykinase	-	Saccharomyces cerevisiae
PEP carboxykinase	-	Anaerobiospirillum succiniciproducens
phosphoenolpyruvate carboxykinase	-	Saccharomyces cerevisiae
phosphoenolpyruvate carboxykinase	-	Anaerobiospirillum succiniciproducens

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Release 2023.1 (January 2023)