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Literature summary for 4.1.1.49 extracted from

  • Chinthapalli, B.; Raghavan, C.; Blasing, O.; Westhoff, P.; Raghavendra, A.S.
    Phosphoenolpyruvate carboxylase purified from leaves of C3, C4, and C3-C4 intermediate species of Alternanthera: Properties at limiting and saturating bicarbonate (2000), Photosynthetica, 38, 415-419.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
D-Glucose-6-phosphate
-
Alternanthera sessilis
D-Glucose-6-phosphate
-
Alternanthera pungens
D-Glucose-6-phosphate
-
Alternanthera ficoides
D-Glucose-6-phosphate
-
Alternanthera ficoidea

Inhibitors

Inhibitors Comment Organism Structure
malate
-
Alternanthera ficoidea
malate
-
Alternanthera ficoides
malate
-
Alternanthera pungens
malate
-
Alternanthera sessilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
phosphoenolpyruvate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera sessilis
0.07
-
phosphoenolpyruvate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera sessilis
0.11
-
phosphoenolpyruvate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera ficoides
0.15
-
phosphoenolpyruvate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera ficoidea
0.2
-
phosphoenolpyruvate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera ficoides
0.28
-
phosphoenolpyruvate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera pungens
0.29
-
phosphoenolpyruvate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera ficoidea
0.62
-
phosphoenolpyruvate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera pungens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 0.05 mM bicarbonate, Km 0.20 mM, 10 mM bicarbonate Km 0.04 mM Alternanthera sessilis
Mg2+ 0.05 mM bicarbonate, Km 0.44 mM, 10 mM bicarbonate Km 0.14 mM Alternanthera ficoides
Mg2+ 0.05 mM bicarbonate, Km 0.50 mM, 10 mM bicarbonate Km 0.15 mM Alternanthera ficoidea
Mg2+ 0.05 mM bicarbonate, Km 1.20 mM, 10 mM bicarbonate Km 0.42 mM Alternanthera pungens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + oxaloacetate Alternanthera sessilis
-
ADP + phosphoenolpyruvate + CO2
-
?
ATP + oxaloacetate Alternanthera pungens
-
ADP + phosphoenolpyruvate + CO2
-
r
ATP + oxaloacetate Alternanthera ficoides
-
ADP + phosphoenolpyruvate + CO2
-
r
ATP + oxaloacetate Alternanthera ficoidea
-
ADP + phosphoenolpyruvate + CO2
-
r

Organism

Organism UniProt Comment Textmining
Alternanthera ficoidea
-
-
-
Alternanthera ficoides
-
-
-
Alternanthera pungens
-
-
-
Alternanthera sessilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Alternanthera sessilis
-
Alternanthera pungens
-
Alternanthera ficoides
-
Alternanthera ficoidea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.5
-
-
Alternanthera pungens

Storage Stability

Storage Stability Organism
-196°C, stored in 50% glycerol in liquid nitrogen, stable for more than 3 months Alternanthera pungens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + oxaloacetate
-
Alternanthera sessilis ADP + phosphoenolpyruvate + CO2
-
?
ATP + oxaloacetate
-
Alternanthera pungens ADP + phosphoenolpyruvate + CO2
-
r
ATP + oxaloacetate
-
Alternanthera ficoides ADP + phosphoenolpyruvate + CO2
-
r
ATP + oxaloacetate
-
Alternanthera ficoidea ADP + phosphoenolpyruvate + CO2
-
r

Synonyms

Synonyms Comment Organism
PEPC
-
Alternanthera sessilis
PEPC
-
Alternanthera pungens
PEPC
-
Alternanthera ficoides
PEPC
-
Alternanthera ficoidea
phosphoenolpyruvate carboxykinase
-
Alternanthera sessilis
phosphoenolpyruvate carboxykinase
-
Alternanthera pungens
phosphoenolpyruvate carboxykinase
-
Alternanthera ficoides
phosphoenolpyruvate carboxykinase
-
Alternanthera ficoidea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.004
-
malate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera sessilis
0.013
-
malate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera ficoides
0.013
-
malate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera sessilis
0.014
-
malate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera ficoidea
0.015
-
malate pH 7.3, 37°C, 0.05 mM bicarbonate Alternanthera pungens
0.046
-
malate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera ficoides
0.052
-
malate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera ficoidea
0.07
-
malate pH 7.3, 37°C, 10 mM bicarbonate Alternanthera pungens