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Literature summary for 4.1.1.48 extracted from

  • Zaccardi, M.J.; Yezdimer, E.M.; Boehr, D.D.
    Functional identification of the general acid and base in the dehydration step of indole-3-glycerol phosphate synthase catalysis (2013), J. Biol. Chem., 288, 26350-26356.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.48

Protein Variants

Protein Variants Comment Organism
E210Q kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is 6fold lower than wild-type value Saccharolobus solfataricus
E51Q kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is 28fold lower than wild-type value Saccharolobus solfataricus
K53Q low activity, kcat is 570fold lower than wild-type value Saccharolobus solfataricus
K53R kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is 500fold lower than wild-type value Saccharolobus solfataricus
R182A kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is 39fold lower than wild-type value Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000013
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme E51Q Saccharolobus solfataricus
0.000044
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, wild-type enzyme Saccharolobus solfataricus
0.000386
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme E210Q Saccharolobus solfataricus
0.00106
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme R182A Saccharolobus solfataricus
0.00244
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme K53R Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q06121
-
-
Saccharolobus solfataricus P2 Q06121
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate Lys53 acts as the general acid in the dehydration step of the reaction Saccharolobus solfataricus 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O
-
 ?
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate Lys53 acts as the general acid in the dehydration step of the reaction Saccharolobus solfataricus P2 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
IGPS
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 assay Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme K53Q Saccharolobus solfataricus
0.007
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme E51Q Saccharolobus solfataricus
0.06
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme K53R Saccharolobus solfataricus
0.41
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme R182A Saccharolobus solfataricus
0.67
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, wild-type enzyme Saccharolobus solfataricus
0.97
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme E210Q Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
30
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme K53R Saccharolobus solfataricus
380
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme R182A Saccharolobus solfataricus
540
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme E51Q Saccharolobus solfataricus
2500
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, mutant enzyme E210Q Saccharolobus solfataricus
15230
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 75°C, wild-type enzyme Saccharolobus solfataricus