Protein Variants | Comment | Organism |
---|---|---|
F246S | about 2fold increase in turnover-number, 8fold increase in KM-value, decrease in kcat/Km | Saccharolobus solfataricus |
G212E | about 2fold increase in turnover-number, 100fold increase Km-value, decrease in kcat/Km. Mutant enzyme is about as thermostable as wild-type enzyme | Saccharolobus solfataricus |
M237T | kcat/Km of 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate is fold than wild-type value | Saccharolobus solfataricus |
P2S | slight increase in kcat/Km (substrate: 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate). Mutant enzyme is about as thermostable as wild-type enzyme | Saccharolobus solfataricus |
P2S/F246S | about 2fold increase in turnover-number, 8fold increase in KM-value, decrease in kcat/Km | Saccharolobus solfataricus |
P2S/G212E | about 4fold increase in turnover-number,200fold increase in Km-valuer, decrease in kcat/Km | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
half-life after trypsinolysis in 0.1 M Tris acetate at pH 7.8 and 25°C: wild-type enzyme (120 min), mutant enzyme P2S (60 min), mutant enzyme F246S (40 min), mutant enzyme G212E (40 min), mutant enzyme P2S/F246S (8 min), mutant enzyme P2S/G212E (15 min) | Saccharolobus solfataricus |
the enzyme is strongly stabilized in phosphate buffer (t1/2: 46 min at 87°C) in comparison to HEPPS buffer (t1/2: 4.4 min at 89°C) | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 25°C, wild-type enzyme | Saccharolobus solfataricus | |
0.00005 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, wild-type enzyme | Saccharolobus solfataricus | |
0.00005 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, mutant enzyme P2S | Saccharolobus solfataricus | |
0.00005 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 60°C, wild-type enzyme | Saccharolobus solfataricus | |
0.0004 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, mutant enzyme F246S | Saccharolobus solfataricus | |
0.00042 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 25°C, mutant enzyme P2S/F246S | Saccharolobus solfataricus | |
0.0056 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, mutant enzyme G212E | Saccharolobus solfataricus | |
0.0109 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 25°C, mutant enzyme P2S/G212E | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Saccharolobus solfataricus | indoleglycerol phosphate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IGP synthase | - |
Saccharolobus solfataricus |
IGPS | - |
Saccharolobus solfataricus |
Indoleglycerol phosphate synthase | - |
Saccharolobus solfataricus |
TrpC | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
86.5 | - |
in 0.05 M potassium phosphate at pH 7.5. Half-life of wild-type enzyme: 46 min, half-life of mutant enzyme P2S: 18 min, hal-life of mutant enzyme F246S 1 min, half-life of mutant enzyme G212E: 38 min, half-life of mutant enzyme P2S/F246S: less than 0.1 min, half-life of mutant enzyme P2S/G212E: 19 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 25°C, wild-type enzyme | Saccharolobus solfataricus | |
0.15 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, wild-type enzyme | Saccharolobus solfataricus | |
0.16 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, mutant enzyme P2S | Saccharolobus solfataricus | |
0.27 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 25°C, mutant enzyme P2S/F246S | Saccharolobus solfataricus | |
0.35 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, mutant enzyme F246S | Saccharolobus solfataricus | |
0.36 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, mutant enzyme G212E | Saccharolobus solfataricus | |
0.57 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 25°C, mutant enzyme P2S/G212E | Saccharolobus solfataricus | |
0.98 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 60°C, wild-type enzyme | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 25°C, mutant enzyme P2S/G212E | Saccharolobus solfataricus | |
100 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, mutant enzyme G212E | Saccharolobus solfataricus | |
600 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 25°C, mutant enzyme P2S/F246S | Saccharolobus solfataricus | |
750 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 25°C, wild-type enzyme | Saccharolobus solfataricus | |
900 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, mutant enzyme F246S | Saccharolobus solfataricus | |
3000 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, wild-type enzyme | Saccharolobus solfataricus | |
3200 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 37°C, mutant enzyme P2S | Saccharolobus solfataricus | |
14200 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 60°C, wild-type enzyme | Saccharolobus solfataricus |