Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
geomycine | 0.5 mg/ml | Mycobacterium tuberculosis | |
H2O2 | 0.001% H2O2 inactivates the enzyme almost completely | Mycobacterium tuberculosis | |
kanamycin | 0.5 mg/ml | Mycobacterium tuberculosis | |
methanol | enzyme activity is gradually lost in the presence of increasing concentrations of methanol and completely lost at 65% | Mycobacterium tuberculosis | |
streptomycin | 0.5 mg/ml | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | significantly affects activity, the optimal concentration is about 0.4-2.0 mM | Mycobacterium tuberculosis | |
Mg2+ | significantly affects activity, the optimal concentration is about 0.4-2.0 mM | Mycobacterium tuberculosis | |
Mn2+ | significantly affects activity, the optimal concentration is about 0.4-2.0 mM | Mycobacterium tuberculosis | |
Na+ | significantly affects activity, the optimal concentration is about 0.4-2.0 mM | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE, recombinant enzyme | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA His-binding resin affinity chromatography | Mycobacterium tuberculosis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:oleate-grown cell | 10% (v/v) oleate-albumin-dextrose complex | Mycobacterium tuberculosis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | - |
recombinant enzyme, at 37°C, pH 7.0 | Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
-70°C, 5 mM Tris-HCl (pH 7.0), at least 2 months, remains stable | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Mycobacterium tuberculosis | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? | |
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Mycobacterium tuberculosis H37Rv | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IGPS | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in 5 mM Tris-HCl buffer | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | little loss of activity after a 10 min heating in a water bath at temperatures from 40 to 50°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
in 5 mM Tris-HCl buffer | Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | in 5 mM Tris-HCl buffer | Mycobacterium tuberculosis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
slight activity at pH 5.0 | Mycobacterium tuberculosis |
8 | - |
almost no activity at pH 8.0 | Mycobacterium tuberculosis |