Crystallization (Comment) | Organism |
---|---|
N-terminal deletion mutant lacking 26 amino acids, structure of core is unchanged compared to wild-type | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of N-terminal 25 amino acids, unchanged oligomerzation states and turnover numbers, increase in Km-value for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate, decrease in resistance towards unfolding induced by heat and guanidinium chloride | Thermotoga maritima |
additional information | deletion of N-terminal 26 amino acids, unchanged oligomerzation states and turnover numbers, increase in Km-value for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate, decrease in resistance towards unfolding induced by heat and guanidinium chloride | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000025 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | wild-type, pH 7.5, 25°C | Thermotoga maritima | |
0.00009 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | wild-type, pH 7.5, 25°C | Saccharolobus solfataricus | |
0.00069 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N-terminal deletion mutant lacking 25 amino acids, pH 7.5, 25°C | Thermotoga maritima | |
0.0099 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N-terminal deletion mutant lacking 26 amino acids, pH 7.5, 25°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23500 | - |
gel filtration, N-terminal deletion mutant lacking 26 amino acids | Saccharolobus solfataricus |
24200 | - |
gel filtration, N-terminal deletion mutant lacking 25 amino acids | Thermotoga maritima |
26300 | - |
gel filtration, wild-type | Saccharolobus solfataricus |
26400 | - |
1 * 26400, N-terminal deletion mutant lacking 26 amino acids, 1 * 28600, wild-type, calculated | Saccharolobus solfataricus |
26600 | - |
and dimer, 1 * 26600, N-terminal deletion mutant lacking 25 amino acids, 1 * 28700, wild-type, calculated | Thermotoga maritima |
26600 | - |
and dimer, 2 * 26600, N-terminal deletion mutant lacking 25 amino acids, 2 * 28700, wild-type, calculated | Thermotoga maritima |
28200 | - |
gel filtration, wild-type | Thermotoga maritima |
28600 | - |
1 * 26400, N-terminal deletion mutant lacking 26 amino acids, 1 * 28600, wild-type, calculated | Saccharolobus solfataricus |
28700 | - |
and dimer, 1 * 26600, N-terminal deletion mutant lacking 25 amino acids, 1 * 28700, wild-type, calculated | Thermotoga maritima |
28700 | - |
and dimer, 2 * 26600, N-terminal deletion mutant lacking 25 amino acids, 2 * 28700, wild-type, calculated | Thermotoga maritima |
39300 | - |
gel filtration, N-terminal deletion mutant lacking 25 amino acids | Thermotoga maritima |
47100 | - |
gel filtration, wild-type | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q06121 | - |
- |
Thermotoga maritima | Q56319 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Saccharolobus solfataricus | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? | |
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Thermotoga maritima | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | and dimer, 2 * 26600, N-terminal deletion mutant lacking 25 amino acids, 2 * 28700, wild-type, calculated | Thermotoga maritima |
monomer | 1 * 26400, N-terminal deletion mutant lacking 26 amino acids, 1 * 28600, wild-type, calculated | Saccharolobus solfataricus |
monomer | and dimer, 1 * 26600, N-terminal deletion mutant lacking 25 amino acids, 1 * 28700, wild-type, calculated | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
half-life of wild-type, 123 min, half-life of N-terminal deletion mutant lacking 26 amino acids, 2.9 min | Saccharolobus solfataricus |
75 | - |
half-life of wild-type, 258 min, half-life of N-terminal deletion mutant lacking 25 amino acids, 6.3 min | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | wild-type, pH 7.5, 25°C | Saccharolobus solfataricus | |
0.028 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N-terminal deletion mutant lacking 26 amino acids, pH 7.5, 25°C | Saccharolobus solfataricus | |
0.06 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N-terminal deletion mutant lacking 25 amino acids, pH 7.5, 25°C | Thermotoga maritima | |
0.095 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | wild-type, pH 7.5, 25°C | Thermotoga maritima |