Literature summary for 4.1.1.48 extracted from

Eberhard, M.; Tsai-Pflugfelder, M.; Bolewska, K.; Hommel, U.; Kirschner, K.
Indoleglycerol phosphate synthase - phosphoribosyl anthranilate isomerase: comparison of the bifunctional enzyme from Escherichia coli with engineered monofunctional domains (1995), Biochemistry, 34, 5419-5428.

Search for more literature for 4.1.1.48

Protein Variants

Protein Variants	Comment	Organism
additional information	engineering of a monofunctional enzyme by separating the domains on the gene level and expression as monofunctional enzymes	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
Reduced 1-[2-carboxyphenyl)amino]-1-deoxyribulose 5-phosphate	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000047	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	genetically engineered monofunctional enzyme form IGPS[1-259]	Escherichia coli
0.00042	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	bifunctional enzyme	Escherichia coli
0.013	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	genetically engineered monofunctional enzyme form IGPS[1-252FAG]	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	bifunctional enzyme phosphoribosylanthranilate isomerase-indoleglycerol phosphate synthetase, Ec4.1.1.48/Ec5.3.1.24	-

Purification (Commentary)

Purification (Comment)	Organism
monofunctional enzyme	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	-	Escherichia coli	1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.2	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	genetically engineered monofunctional enzyme form IGPS[1-259]	Escherichia coli
3.6	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	bifunctional enzyme	Escherichia coli
4.3	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	genetically engineered monofunctional enzyme form IGPS[1-252FAG]	Escherichia coli

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Release 2023.1 (January 2023)