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Literature summary for 4.1.1.45 extracted from

  • Huo, L.; Davis, I.; Chen, L.; Liu, A.
    The power of two: arginine 51 and arginine 239* from a neighboring subunit are essential for catalysis in alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (2013), J. Biol. Chem., 288, 30862-30871.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.45

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
R239A catalytically inactive Pseudomonas fluorescens
R239K catalytically inactive Pseudomonas fluorescens
R51A catalytically inactive Pseudomonas fluorescens
R51K catalytically inactive Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
pyridine-2,6-dicarboxylic acid competitive Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate wild type enzyme, at pH 7.0 and 37°C Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate Pseudomonas fluorescens
-
 2-aminomuconate semialdehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Superdex 75 gel filtration Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate
-
 Pseudomonas fluorescens 2-aminomuconate semialdehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Pseudomonas fluorescens

Synonyms

Synonyms Comment Organism
ACMSD
-
 Pseudomonas fluorescens
alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase
-
 Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5
-
 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate wild type enzyme, at pH 7.0 and 22°C Pseudomonas fluorescens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0227
-
 pyridine-2,6-dicarboxylic acid wild type enzyme, at pH 7.0 and 22°C Pseudomonas fluorescens