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Literature summary for 4.1.1.43 extracted from

  • Spaepen, S.; Versees, W.; Gocke, D.; Pohl, M.; Steyaert, J.; Vanderleyden, J.
    Characterization of phenylpyruvate decarboxylase, involved in auxin production of Azospirillum brasilense (2007), J. Bacteriol., 189, 7626-7633.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.43

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RP cells Azospirillum brasilense

Protein Variants

Protein Variants Comment Organism
additional information an ipdC-knockout mutant produces only 10% (w/v) of the wild type indole-3-acetic acid production level Azospirillum brasilense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 Indole-3-pyruvate in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
0.3
-
 5-phenyl-2-oxopentanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
1.08
-
 phenylpyruvate in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
1.91
-
 4-phenyl-2-oxobutanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
5.51
-
 2-ketohexanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 4 * 60000, gel filtration Azospirillum brasilense
240600
-
 gel filtration, recombinant enzyme Azospirillum brasilense

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP column chromatography and Superdex G200 gel filtration Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ketohexanoic acid 1% activity compared to phenylpyruvate Azospirillum brasilense pentanal + CO2
-
 ?
4-phenyl-2-oxobutanoic acid 2.3% activity compared to phenylpyruvate Azospirillum brasilense 3-phenylpropanal + CO2
-
 ?
5-phenyl-2-oxopentanoic acid 110% activity compared to phenylpyruvate Azospirillum brasilense 4-phenylbutanal + CO2
-
 ?
indole-3-pyruvate 10.4% activity compared to phenylpyruvate Azospirillum brasilense indoleacetaldehyde + CO2
-
 ?
additional information no activity with benzoylformate and indole-glyoxylic acid Azospirillum brasilense ?
-
 ?
Phenylpyruvate 100% activity Azospirillum brasilense Phenylacetaldehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 60000, gel filtration Azospirillum brasilense

Synonyms

Synonyms Comment Organism
phenylpyruvate decarboxylase formerly classified as indole-3-pyruvate decarboxylase (ipdC) Azospirillum brasilense

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 phenylpyruvate in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
0.07
-
 Indole-3-pyruvate in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
0.21
-
 4-phenyl-2-oxobutanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
0.29
-
 2-ketohexanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
1.7
-
 5-phenyl-2-oxopentanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
5.56
-
 phenylpyruvate in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate 0.1 mM Azospirillum brasilense