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Literature summary for 4.1.1.4 extracted from

  • Highbarger, L.A.; Gerlt, J.A.; Kenyon, G.L.
    Mechanism of the reaction catalyzed by acetoacetate decarboxylase. Importance of lysine 116 in determining the pKa of the active-site lysine 115 (1996), Biochemistry, 35, 41-46.
    View publication on PubMed
Search for more literature for 4.1.1.4

Protein Variants

Protein Variants Comment Organism
K115C mutant enzymes K115C and K115Q are catalytically inactive at pH 5.95. Mutant enzymes K116C, K116N and K116R have reduced but significant activities Clostridium acetobutylicum
K115Q mutant enzymes K115C and K115Q are catalytically inactive at pH 5.95. Mutant enzymes K116C, K116N and K116R have reduced but significant activities Clostridium acetobutylicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.2
-
 acetoacetate wild-type enzyme Clostridium acetobutylicum
8.4
-
 acetoacetate mutant enzyme K116C Clostridium acetobutylicum
10
-
 acetoacetate mutant enzyme K116N Clostridium acetobutylicum
14.7
-
 acetoacetate mutant enzyme K116R Clostridium acetobutylicum

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum
-
 wild-type and mutant enzymes K115C, K115Q, K116C, K116N and K116R
-

Reaction

Reaction Comment Organism Reaction ID
acetoacetate = acetone + CO2 mechanism Clostridium acetobutylicum
a
acetoacetate = acetone + CO2 Schiff base intermediate Clostridium acetobutylicum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acetoacetate
-
 Clostridium acetobutylicum Acetone + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30
-
 acetoacetate mutant enzyme K116N Clostridium acetobutylicum
38
-
 acetoacetate mutant enzyme K116C Clostridium acetobutylicum
302
-
 acetoacetate mutant enzyme K116R Clostridium acetobutylicum
1560
-
 acetoacetate wild-type enzyme Clostridium acetobutylicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 wild-type enzyme Clostridium acetobutylicum