Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
M330L | increase of Km for carboxylase and oxygenase reaction | Rhodospirillum rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
D-ribulose 1,5-bisphosphate | wild-type enzyme | Rhodospirillum rubrum | |
0.16 | - |
CO2 | wild-type enzyme | Rhodospirillum rubrum | |
0.17 | - |
O2 | wild-type enzyme | Rhodospirillum rubrum | |
1.4 | - |
D-ribulose 1,5-bisphosphate | mutant Met330Leu | Rhodospirillum rubrum | |
2.35 | - |
CO2 | mutant Met330Leu | Rhodospirillum rubrum | |
6 | - |
O2 | mutant Met330Leu | Rhodospirillum rubrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ribulose 1,5-bisphosphate + CO2 | Rhodospirillum rubrum | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodospirillum rubrum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O | D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction | Rhodospirillum rubrum | |
2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O | D-Ribulose 1,5-bisphosphate + O2 = D-3-phosphoglycerate + 2-phosphoglycolate, oxygenase reaction | Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ribulose 1,5-bisphosphate + CO2 | - |
Rhodospirillum rubrum | D-3-Phosphoglycerate | - |
? | |
D-Ribulose 1,5-bisphosphate + CO2 | - |
Rhodospirillum rubrum | ? | - |
? | |
D-Ribulose 1,5-bisphosphate + O2 | - |
Rhodospirillum rubrum | D-3-Phosphoglycerate + 2-phosphoglycolate | - |
? |