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Literature summary for 4.1.1.33 extracted from

  • Krepkiy, D.; Miziorko, H.M.
    Identification of active site residues in mevalonate diphosphate decarboxylase: implications for a family of phosphotransferases (2004), Protein Sci., 13, 1875-1881.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.33

Protein Variants

Protein Variants Comment Organism
D302A 1000fold decrease in kcat value, still retains ability to stoichiometrically bind nucleotide triphosphates at the active site Saccharomyces cerevisiae
D302N 100000fold decrease in kcat value, still retains ability to stoichiometrically bind nucleotide triphosphates at the active site Saccharomyces cerevisiae
K18M 30fold decrease in kcat value, still retains ability to stoichiometrically bind nucleotide triphosphates at the active site Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 ATP wild-type, pH 7.0, 30°C Saccharomyces cerevisiae
0.103
-
 5-diphosphomevalonate mutant D302N, pH 7.0, 30°C Saccharomyces cerevisiae
0.123
-
 5-diphosphomevalonate wild-type, pH 7.0, 30°C Saccharomyces cerevisiae
0.177
-
 5-diphosphomevalonate mutant K18M, pH 7.0, 30°C Saccharomyces cerevisiae
0.397
-
 5-diphosphomevalonate mutant D302A, pH 7.0, 30°C Saccharomyces cerevisiae
1.066
-
 ATP mutant K18M, pH 7.0, 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE Saccharomyces cerevisiae
107000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P32377
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4
-
 pH 7.0, 30°C, recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-diphosphomevalonate
-
 Saccharomyces cerevisiae ADP + phosphate + isopentenyl diphosphate + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 5-diphosphomevalonate mutant K18M, pH 7.0, 30°C Saccharomyces cerevisiae
4.9
-
 5-diphosphomevalonate wild-type, pH 7.0, 30°C Saccharomyces cerevisiae