Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GMP | competitive inhibitor | Raillietina echinobothrida | |
GTP | competitive inhibitor | Raillietina echinobothrida | |
IMP | competitive inhibitor | Raillietina echinobothrida | |
ITP | competitive inhibitor | Raillietina echinobothrida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02412 | - |
phosphoenolpyruvate | with IDP as cosubstrate, in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
0.02516 | - |
GDP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
0.04252 | - |
phosphoenolpyruvate | with GDP as cosubstrate, in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
0.1273 | - |
IDP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
4.76 | - |
CO2 | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required, 4 mM used in assay conditions | Raillietina echinobothrida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
high pressure liquid chromatography | Raillietina echinobothrida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + phosphoenolpyruvate + CO2 | Raillietina echinobothrida | highest activity | GTP + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Raillietina echinobothrida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephacel column chromatography, and Blue Sepharose CL 6B affinity chromatography | Raillietina echinobothrida |
Storage Stability | Organism |
---|---|
-80°C, more than 1 year, the enzyme remains stable | Raillietina echinobothrida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + phosphoenolpyruvate + CO2 | highest activity | Raillietina echinobothrida | GTP + oxaloacetate | - |
? | |
IDP + phosphoenolpyruvate + CO2 | lower activity compared to GDP | Raillietina echinobothrida | ITP + oxaloacetate | - |
? | |
additional information | no enzyme activity observed when ADP or CDP is used | Raillietina echinobothrida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, SDS-PAGE | Raillietina echinobothrida |
Synonyms | Comment | Organism |
---|---|---|
PEP carboxykinase | - |
Raillietina echinobothrida |
PEPCK | - |
Raillietina echinobothrida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Raillietina echinobothrida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8 | there is a steep rise in the enzyme activity (about 2fold) between the pH 6.6 and pH 7.4, although a sharp decline is observed within a narrow range (pH 7.48.0) | Raillietina echinobothrida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
GDP | - |
Raillietina echinobothrida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07642 | - |
GTP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
0.1864 | - |
GMP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
0.2264 | - |
ITP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida | |
0.3483 | - |
IMP | in Tris-HCl (50 mM, pH 7.4), temperature not specified in the publication | Raillietina echinobothrida |