Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.32 extracted from

  • Dharmarajan, L.; Case, C.L.; Dunten, P.; Mukhopadhyay, B.
    Tyr235 of human cytosolic phosphoenolpyruvate carboxykinase influences catalysis through an anion-quadrupole interaction with phosphoenolpyruvate carboxylate (2008), FEBS J., 275, 5810-5819.
    View publication on PubMed
Search for more literature for 4.1.1.32

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y235A site-directed mutagenesis Homo sapiens
Y235F site-directed mutagenesis Homo sapiens
Y235S site-directed mutagenesis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 GTP Y235F mutant, Vmax = 41 micromol/min/mg Homo sapiens
0.033
-
 oxaloacetate wild-type, Vmax = 35 micromol/min/mg Homo sapiens
0.034
-
 GDP Mg2+ concentration 2 mM, Y235A mutant. Vmax = 15 micromol/min/mg Homo sapiens
0.034
-
 GDP Mg2+ concentration 7.5 mM, Y235A mutant. Vmax = 15 micromol/min/mg Homo sapiens
0.035
-
 GDP Mg2+ concentration 5 mM, Y235A mutant. Vmax = 15 micromol/min/mg Homo sapiens
0.036
-
 phosphoenolpyruvate Mn2+ concentration 1.5 mM, Mg2+ concentration 2 mM, Y235F mutant. Vmax = 3 micromol/min/mg Homo sapiens
0.038
-
 oxaloacetate Y235F mutant, Vmax = 39 micromol/min/mg Homo sapiens
0.04
-
 GDP Mg2+ concentration 5 mM, Y235S mutant. Vmax = 10 micromol/min/mg Homo sapiens
0.041
-
 GDP Mg2+ concentration 7.5 mM, wild-type. Vmax = 35 micromol/min/mg Homo sapiens
0.043
-
 GTP Y235S mutant, Vmax = 3 micromol/min/mg Homo sapiens
0.044
-
 GDP Mg2+ concentration 2 mM, Y235S mutant. Vmax = 11 micromol/min/mg Homo sapiens
0.044
-
 GDP Mg2+ concentration 5 mM, wild-type. Vmax = 35 micromol/min/mg Homo sapiens
0.045
-
 GDP Mg2+ concentration 2 mM, wild-type. Vmax = 33 micromol/min/mg Homo sapiens
0.047
-
 GDP Mg2+ concentration 2 mM, Y235F mutant. Vmax = 3 micromol/min/mg Homo sapiens
0.047
-
 oxaloacetate Y235A mutant, Vmax = 3 micromol/min/mg Homo sapiens
0.053
-
 GDP Mg2+ concentration 7.5 mM, Y235S mutant. Vmax = 11 micromol/min/mg Homo sapiens
0.059
-
 GTP Y235A mutant, Vmax = 3 micromol/min/mg Homo sapiens
0.06
-
 GDP Mg2+ concentration 5 mM, Y235F mutant. Vmax = 3 micromol/min/mg Homo sapiens
0.061
-
 oxaloacetate Y235S mutant, Vmax = 3 micromol/min/mg Homo sapiens
0.064
-
 GTP wild-type, Vmax = 39 micromol/min/mg Homo sapiens
0.067
-
 GDP Mg2+ concentration 7.5 mM, Y235F mutant. Vmax = 3 micromol/min/mg Homo sapiens
0.217
-
 phosphoenolpyruvate Mn2+ concentration 1.5 mM, Mg2+ concentration 2 mM, wild-type. Vmax = 31 micromol/min/mg Homo sapiens
0.919
-
 phosphoenolpyruvate Mn2+ concentration 1.5 mM, Mg2+ concentration 2 mM, Y235A mutant. Vmax = 20 micromol/min/mg Homo sapiens
1.256
-
 phosphoenolpyruvate Mn2+ concentration 1.5 mM, Mg2+ concentration 2 mM, Y235S mutant. Vmax = 15 micromol/min/mg Homo sapiens
6.8
-
 CO2 Mg2+ concentration 2 mM, Y235A mutant. Vmax = 21 micromol/min/mg Homo sapiens
6.8
-
 CO2 CO2 in form of HCO3- Homo sapiens
7.2
-
 CO2 Mg2+ concentration 2 mM, Y235F mutant. Vmax = 5 micromol/min/mg Homo sapiens
7.2
-
 CO2 CO2 in form of HCO3- Homo sapiens
7.5
-
 CO2 Mg2+ concentration 2 mM, Y235S mutant. Vmax = 11 micromol/min/mg Homo sapiens
7.5
-
 CO2 CO2 in form of HCO3- Homo sapiens
20.7
-
 CO2 Mg2+ concentration 2 mM, wild-type. Vmax = 44 micromol/min/mg Homo sapiens
20.7
-
 CO2 CO2 in form of HCO3- Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the IC50 values for Mn2+ are 0.009 mM for Mg2+ concentration 2 mM, wild-type, Vmax = 31 micromol/min/mg, 0.607 mM for Mg2+ concentration 2 mM, Y235F mutant, Vmax = 4 micromol/min/mg, 0.073 mM for Mg2+ concentration 2 mM, Y235A mutant, Vmax = 19 micromol/min/mg, 0.058 mM for Mg2+ concentration 2 mM, Y235S mutant, Vmax = 13 micromol/min/mg, 0.0008 mM for wild-type, Vmax = 29 micromol/min/mg, 0.0007 mM for Y235F mutant, Vmax = 31 micromol/min/mg, 0.0004 mM for Y235A mutant, Vmax = 2 micromol/min/mg, 0.0007 mM for Y235S mutant, Vmax = 2 micromol/min/mg, 0.009 mM for wild-type, Mg2+ concentration 5 mM, Vmax = 31 micromol/min/mg, 0.562 mM for Y235F mutant, Mg2+ concentration 5 mM, Vmax = 4 micromol/min/mg, 0.064 mM for Y235A mutant, Mg2+ concentration 5 mM, Vmax = 18 micromol/min/mg, 0.048 mM for Y235S mutant, Mg2+ concentration 5 mM, Vmax = 13 micromol/min/mg, 0.009 mM for wild-type, Mg2+ concentration 7.5 mM, Vmax = 31 micromol/min/mg, 0.521 mM for Y235F mutant, Mg2+ concentration 7.5 mM, Vmax = 4 micromol/min/mg, 0.057 mM for Y235A mutant, Mg2+ concentration 7.5 mM, Vmax = 18 micromol/min/mg, 0.043 mM for Y235S mutant, Mg2+ concentration 7.5 mM, Vmax = 13 micromol/min/mg Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68500
-
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P35558
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + phosphoenolpyruvate + CO2
-
 Homo sapiens GTP + oxaloacetate
-
 ?

Synonyms

Synonyms Comment Organism
PEP carboxykinase
-
 Homo sapiens
PEPCK
-
 Homo sapiens
phosphoenolpyruvate carboxykinase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
GTP
-
 Homo sapiens