Literature summary for 4.1.1.32 extracted from

Case, C.L.; Concar, E.M.; Boswell, K.L.; Mukhopadhyay, B.
Roles of Asp75, Asp78, and Glu83 of GTP-dependent phosphoenolpyruvate carboxykinase from Mycobacterium smegmatis (2006), J. Biol. Chem., 281, 39262-39272.

Search for more literature for 4.1.1.32

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli C41(DE3) cells	Mycolicibacterium smegmatis

Protein Variants

Protein Variants	Comment	Organism
D75A	75% reduced activity	Mycolicibacterium smegmatis
D75N	reduced activity	Mycolicibacterium smegmatis
D75Q	reduced activity	Mycolicibacterium smegmatis
D75S	reduced activity	Mycolicibacterium smegmatis
D78A	severely reduced activity	Mycolicibacterium smegmatis
E83A	severely reduced activity	Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0067	-	oxaloacetate	wild type enzyme	Mycolicibacterium smegmatis
0.0115	-	oxaloacetate	mutant enzyme E83A	Mycolicibacterium smegmatis
0.0138	-	oxaloacetate	mutant enzyme D75N	Mycolicibacterium smegmatis
0.018	-	oxaloacetate	mutant enzyme D75Q	Mycolicibacterium smegmatis
0.0183	-	oxaloacetate	mutant enzyme D75A	Mycolicibacterium smegmatis
0.0189	-	oxaloacetate	mutant enzyme D75S	Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
71200	-	calculated from the nucleic acid sequence-derived amino acid sequence	Mycolicibacterium smegmatis

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	Q9AGJ6	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.06	-	mutant enzyme D78A, phosphoenolpyruvate-forming activity in the presence of 0.2 mM Mn2+	Mycolicibacterium smegmatis
0.13	-	mutant enzyme D78A, oxaloacetate-forming activity in the presence of 0.2 mM Mn2+	Mycolicibacterium smegmatis
0.15	-	mutant enzyme D78A, oxaloacetate-forming activity in the presence of 2 mM Mn2+	Mycolicibacterium smegmatis
0.36	-	mutant enzyme E83A, phosphoenolpyruvate-forming activity in the presence of 0.2 mM Mn2+	Mycolicibacterium smegmatis
0.4	-	mutant enzyme E83A, oxaloacetate-forming activity in the presence of 0.2 mM Mn2+	Mycolicibacterium smegmatis
1	-	mutant enzyme E83A, oxaloacetate-forming activity in the presence of 2 mM Mn2+	Mycolicibacterium smegmatis
10.6	-	mutant enzyme D75A, oxaloacetate-forming activity in the presence of 0.2 mM Mn2+	Mycolicibacterium smegmatis
12.3	-	wild type enzyme, phosphoenolpyruvate-forming activity in the presence of 0.2 mM Mn2+	Mycolicibacterium smegmatis
15.5	-	mutant enzyme D75A, phosphoenolpyruvate-forming activity in the presence of 0.2 mM Mn2	Mycolicibacterium smegmatis
29.4	-	wild type enzyme, oxaloacetate-forming activity in the presence of 2 mM Mn2+	Mycolicibacterium smegmatis
43	-	wild type enzyme, oxaloacetate-forming activity in the presence of 0.2 mM Mn2+	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + oxaloacetate	-	Mycolicibacterium smegmatis	GDP + phosphoenolpyruvate + CO2	-	r

Synonyms

Synonyms	Comment	Organism
GTP-PEPCK	-	Mycolicibacterium smegmatis
PEP carboxykinase	-	Mycolicibacterium smegmatis
phosphoenolpyruvate carboxykinase, GTP-dependent	-	Mycolicibacterium smegmatis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)