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Literature summary for 4.1.1.32 extracted from
- Expression, purification, and characterization of a bacterial GTP-dependent PEP carboxykinase (2003), Protein Expr. Purif., 31, 298-304.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| pckA gene, overexpression in Escherichia coli HG89 | Corynebacterium glutamicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cr3+ | - |
Corynebacterium glutamicum |  |
| additional information | not inhibited by dithiothreitol | Corynebacterium glutamicum | |
| Zn2+ | - |
Corynebacterium glutamicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for NaHCO3: 5.48 mM | Corynebacterium glutamicum | |
| 0.05 | - |
GDP | pH 6.8 | Corynebacterium glutamicum | |
| 0.41 | - |
phosphoenolpyruvate | pH 6.8 | Corynebacterium glutamicum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | recombinant enzyme | Corynebacterium glutamicum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | sligthly activates | Corynebacterium glutamicum | |
| Co2+ | significantly activates | Corynebacterium glutamicum | |
| Mg2+ | absolute requirement of a metal ion, activity is maximal in the presence of both Mn2+ and Mg2+, for single ion activation Mn2+ can be substituted by Mg2+ with a reduced enzyme activity | Corynebacterium glutamicum | |
| Mn2+ | absolute requirement of a metal ion, activity is maximal in the presence of both Mn2+ and Mg2+, for single ion activation Mn2+ can be substituted by Mg2+ with a reduced enzyme activity | Corynebacterium glutamicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67512 | - |
1 * 67512, calculated from the amino acid sequence | Corynebacterium glutamicum |
| 67540 | - |
recombinant enzyme, mass spectrometry | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + oxaloacetate | Corynebacterium glutamicum | key rate-limiting step in the gluconeogenic pathway, plays a role in the production of glutamine and lysine through the TCA cycle intermediates | GDP + phosphoenolpyruvate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 7.5fold, recombinant enzyme | Corynebacterium glutamicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + oxaloacetate | GTP-specific | Corynebacterium glutamicum | GDP + phosphoenolpyruvate + CO2 | - |
r | |
| GTP + oxaloacetate | key rate-limiting step in the gluconeogenic pathway, plays a role in the production of glutamine and lysine through the TCA cycle intermediates | Corynebacterium glutamicum | GDP + phosphoenolpyruvate + CO2 | - |
? | |
| ITP + oxaloacetate | lower activity with IDP than with GDP in the reverse reaction | Corynebacterium glutamicum | IDP + phosphoenolpyruvate + CO2 | - |
r | |
| additional information | reverse reaction: not ADP | Corynebacterium glutamicum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 67512, calculated from the amino acid sequence | Corynebacterium glutamicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at room temperature | Corynebacterium glutamicum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kcat for NaHCO3: 6.7 s-1 | Corynebacterium glutamicum | |
| 6.1 | - |
phosphoenolpyruvate | pH 6.8 | Corynebacterium glutamicum | |
| 7.2 | - |
GDP | pH 6.8 | Corynebacterium glutamicum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Corynebacterium glutamicum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Corynebacterium glutamicum | isoelectric focusing | - |
4.5 |
| Corynebacterium glutamicum | theoretically calculated pI | - |
4.61 |
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