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Literature summary for 4.1.1.32 extracted from

  • Dunten, P.; Belunis, C.; Crowther, R.; Hollfelder, K.; Kammlott, U.; Levin, W.; Michel, H.; Ramsey, G.B.; Swain, A.; Weber, D.; Wertheimer, S.J.
    Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site (2002), J. Mol. Biol., 316, 257-264.
    View publication on PubMed

Application

Application Comment Organism
medicine inhibitors of PEPCK may be useful in the treatment of type II diabetes Homo sapiens
pharmacology development of a PEPCK inhibitor may lead to a new therapeutic strategy for the treatment of type II diabetes Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, sequencing Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme alone and in complexes with the non-hydrolyzable GTP analog beta,gamma-methylene GTP, and with phosphoenolpyruvate, hanging-drop vapor-diffusion method, X-ray analysis Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ active site-bound, mode of binding Homo sapiens
Mn2+ active site-bound, mode of binding Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69448
-
x * 69448, recombinant enzyme, mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + oxaloacetate Homo sapiens catalyzes the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle GDP + phosphoenolpyruvate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + oxaloacetate GTP-dependent, enzyme and GTP-binding site structure Homo sapiens GDP + phosphoenolpyruvate + CO2 phosphoenolpyruvate binds as a 1:1 complex with Na+, structure of the phosphoenolpyruvate-binding site r
GTP + oxaloacetate catalyzes the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle Homo sapiens GDP + phosphoenolpyruvate + CO2
-
?

Subunits

Subunits Comment Organism
? x * 69448, recombinant enzyme, mass spectrometry Homo sapiens