Application | Comment | Organism |
---|---|---|
medicine | inhibitors of PEPCK may be useful in the treatment of type II diabetes | Homo sapiens |
pharmacology | development of a PEPCK inhibitor may lead to a new therapeutic strategy for the treatment of type II diabetes | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, sequencing | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
enzyme alone and in complexes with the non-hydrolyzable GTP analog beta,gamma-methylene GTP, and with phosphoenolpyruvate, hanging-drop vapor-diffusion method, X-ray analysis | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | active site-bound, mode of binding | Homo sapiens | |
Mn2+ | active site-bound, mode of binding | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69448 | - |
x * 69448, recombinant enzyme, mass spectrometry | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + oxaloacetate | Homo sapiens | catalyzes the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle | GDP + phosphoenolpyruvate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + oxaloacetate | GTP-dependent, enzyme and GTP-binding site structure | Homo sapiens | GDP + phosphoenolpyruvate + CO2 | phosphoenolpyruvate binds as a 1:1 complex with Na+, structure of the phosphoenolpyruvate-binding site | r | |
GTP + oxaloacetate | catalyzes the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle | Homo sapiens | GDP + phosphoenolpyruvate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 69448, recombinant enzyme, mass spectrometry | Homo sapiens |