Application | Comment | Organism |
---|---|---|
pharmacology | orally active compounds reversibly inhibiting PEPCK improve glucose homeostasis in type 2 diabetics | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-allyl-3-butyl-8-methylxanthine | weak, competitive inhibitor with respect to GTP, IC50: 0.225 mM in the decarboxylation direction, modifications at N-1 and C-8 improve the inhibitory activity by more than 100fold | Homo sapiens | |
3-Mercaptopicolinic acid | reversible, non-competitive inhibitor, IC50: in the 0.02 mM range | Homo sapiens | |
additional information | study of several modified 3-alkyl-1,8-dibenzylxanthines as competitive inhibitors with respect to GTP, IC50-values | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | Mn2+-dependent | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + oxaloacetate | Homo sapiens | catalyzes the rate-limiting step in gluconeogenesis | GDP + phosphoenolpyruvate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + oxaloacetate | GTP-dependent | Homo sapiens | GDP + phosphoenolpyruvate + CO2 | - |
r | |
GTP + oxaloacetate | catalyzes the rate-limiting step in gluconeogenesis | Homo sapiens | GDP + phosphoenolpyruvate + CO2 | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.02 | - |
reversible, non-competitive inhibitor, IC50: in the 0.02 mM range | Homo sapiens | 3-Mercaptopicolinic acid | |
0.225 | - |
weak, competitive inhibitor with respect to GTP, IC50: 0.225 mM in the decarboxylation direction, modifications at N-1 and C-8 improve the inhibitory activity by more than 100fold | Homo sapiens | 1-allyl-3-butyl-8-methylxanthine |