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Literature summary for 4.1.1.32 extracted from

  • Holyoak, T.; Nowak, T.
    Structural investigation of the binding of nucleotide to phosphoenolpyruvate carboxykinase by NMR (2001), Biochemistry, 40, 11037-11047.
    View publication on PubMed
Search for more literature for 4.1.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL-21 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information mechanism of inactivation of PEPCK by Cys-273 modification due to inhibition of a dynamic motion that may occur upon nucleotide binding Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic isoform Rattus norvegicus 5829
-
mitochondrion mitochondrial isoform Gallus gallus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ binds to enzyme more weakly than Mn2+ Rattus norvegicus
Mn2+ binds to the active site, activates Gallus gallus
Mn2+ binds to the active site, activates Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 1 * 67000 Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + oxaloacetate Gallus gallus primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis GDP + phosphoenolpyruvate + CO2
-
 ?
GTP + oxaloacetate Rattus norvegicus primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis GDP + phosphoenolpyruvate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus
recombinant enzyme Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + oxaloacetate specifically requires GTP or ITP, structural data of GTP binding Gallus gallus GDP + phosphoenolpyruvate + CO2
-
 r
GTP + oxaloacetate specifically requires GTP or ITP, structural data of GTP binding Rattus norvegicus GDP + phosphoenolpyruvate + CO2
-
 r
GTP + oxaloacetate primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis Gallus gallus GDP + phosphoenolpyruvate + CO2
-
 ?
GTP + oxaloacetate primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis Rattus norvegicus GDP + phosphoenolpyruvate + CO2
-
 ?
ITP + oxaloacetate specifically requires GTP or ITP Gallus gallus IDP + phosphoenolpyruvate + CO2
-
 r
ITP + oxaloacetate specifically requires GTP or ITP Rattus norvegicus IDP + phosphoenolpyruvate + CO2
-
 r

Subunits

Subunits Comment Organism
monomer
-
 Rattus norvegicus
monomer 1 * 67000 Gallus gallus

Synonyms

Synonyms Comment Organism
GTP/ITP: oxaloacetate carboxylase (transphosphorylating)
-
 Gallus gallus
GTP/ITP: oxaloacetate carboxylase (transphosphorylating)
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Gallus gallus
25
-
 assay at Rattus norvegicus