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Literature summary for 4.1.1.32 extracted from

  • Zeitouni, N.; Eubank, D.W.; Lee, A.Q.; Oxford, M.G.; Freeman, T.L.; Mailliard, M.E.; Beale, E.G.
    Phosphoenolpyruvate carboxykinase is induced in growth-arrested hepatoma cells (2002), Biochem. Biophys. Res. Commun., 290, 1513-1520.
    View publication on PubMed
Search for more literature for 4.1.1.32

Activating Compound

Activating Compound Comment Organism Structure
additional information PEPCK mRNA is elevated in cells cultured at high density, expression is induced by contact inhibition in the presence of serum, at low culture density, dexamethasone induces PEPCK mRNA 5-8fold, at high culture density 2-3fold Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information insulin is a potent dominant repressor of PEPCK gene transcription and causes a rapid decrease of PEPCK mRNA Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + oxaloacetate Rattus norvegicus catalyzes the rate-limiting step in gluconeogenesis, PEPCK expression and growth arrest may be coordinately regulated GDP + phosphoenolpyruvate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hepatoma cell line H4IIEC3 cells, mRNA is 5-10fold elevated in cells cultured at high density compared to low density, KRC-7 cells, an H4IIEC3 subclone, are much more sensitive to growth arrest than its parental cell line Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + oxaloacetate
-
 Rattus norvegicus GDP + phosphoenolpyruvate + CO2
-
 ?
GTP + oxaloacetate catalyzes the rate-limiting step in gluconeogenesis, PEPCK expression and growth arrest may be coordinately regulated Rattus norvegicus GDP + phosphoenolpyruvate + CO2
-
 ?
ITP + oxaloacetate
-
 Rattus norvegicus IDP + phosphoenolpyruvate + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus