Any feedback?
Literature summary for 4.1.1.32 extracted from
- Phosphoenolpyruvate carboxykinase (guanosine triphosphate) from rat liver cytosol. Separation of homogenous forms of the enzyme with high and low activity by chromatography on agarose-hexane-guanosine triphosphate (1978), Biochemistry, 17, 5321-5329.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 5 mM, 19% inhibition | Rattus norvegicus |  |
| EDTA | 0.5 mM, 86% inhibition | Rattus norvegicus | |
| FeCl2 | 1.5 mM FeCl2 causes rapid inactivation. 0.05 mM FeCl2 and 0.0044 mg/ml ferroactivator, enhance to 3.1times the unstimulated rate | Rattus norvegicus | |
| MgCl2 | 1.5 mM, 62% inhibition | Rattus norvegicus | |
| Mn2+ | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0075 | - |
GDP | - |
Rattus norvegicus | |
| 0.0211 | - |
GTP | - |
Rattus norvegicus | |
| 0.026 | - |
oxaloacetate | - |
Rattus norvegicus | |
| 0.048 | - |
phosphoenolpyruvate | - |
Rattus norvegicus | |
| 0.0715 | - |
IDP | - |
Rattus norvegicus | |
| 0.279 | - |
ITP | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 0.05 mM FeCl2 and 0.0044 mg/ml ferroactivator, enhance to 3.1times the unstimulated rate. 1.5 mM FeCl2 causes rapid inactivation | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71500 | - |
1 * 71500, SDS-PAGE | Rattus norvegicus |
| 74000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.7 | - |
carboxylation of phosphoenolpyruvate | Rattus norvegicus |
| 19.9 | - |
decarboxylation of oxaloacetate | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in absence of metal ions, under N2, protein concentration 0.5 mg/ml or higher, stable for at least 14 days | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP + phosphoenolpyruvate + CO2 | - |
Rattus norvegicus | GTP + oxaloacetate | - |
? | |
| GTP + oxaloacetate | - |
Rattus norvegicus | GDP + phosphoenolpyruvate + CO2 | - |
? | |
| IDP + phosphoenolpyruvate + CO2 | - |
Rattus norvegicus | ITP + oxaloacetate | - |
? | |
| ITP + oxaloacetate | - |
Rattus norvegicus | IDP + phosphoenolpyruvate + CO2 | - |
? | |
| oxaloacetate + ? | ir | Rattus norvegicus | pyruvate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 71500, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
protein concentration 0.057 mg/ml, glutathione concentration 0.2 mM, stable for at least 3 h | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
