Literature summary for 4.1.1.31 extracted from

Nagano-Shoji, M.; Hamamoto, Y.; Mizuno, Y.; Yamada, A.; Kikuchi, M.; Shirouzu, M.; Umehara, T.; Yoshida, M.; Nishiyama, M.; Kosono, S.
Characterization of lysine acetylation of a phosphoenolpyruvate carboxylase involved in glutamate overproduction in Corynebacterium glutamicum (2017), Mol. Microbiol., 104, 677-689 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Corynebacterium glutamicum

Protein Variants

Protein Variants	Comment	Organism
K112E	mutation at succinylation site, does not affect glutamate production	Corynebacterium glutamicum
K112R	mutation at succinylation site, does not affect glutamate production	Corynebacterium glutamicum
K653Q	acetylation mimic mutation, abolishes glutamate production. Growth is not affected	Corynebacterium glutamicum
K653R	non-acylation mimic mutant, retains glutamate production, the final glutamate concentration does not reach wild-type levels. Growth is not affected	Corynebacterium glutamicum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.7	-	phosphoenolpyruvate	wild-type, pH 8.5, 25°C	Corynebacterium glutamicum
8.8	-	phosphoenolpyruvate	mutant K653R, pH 8.5, 25°C	Corynebacterium glutamicum
22.2	-	phosphoenolpyruvate	mutant K653Q, pH 8.5, 25°C	Corynebacterium glutamicum

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	P12880	-	-
Corynebacterium glutamicum DSM 20300	P12880	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
acylation	acetylation of PEPC at lysine 653 decreases enzymatic activity, leading to reduced glutamate production. NCgl0616, a sirtuin-type deacetylase, deacetylates K653-acetylated PEPC in vitro	Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.7	-	mutant K653Q, pH 8.5, 25°C	Corynebacterium glutamicum
9.4	-	mutant K653R, pH 8.5, 25°C	Corynebacterium glutamicum
23.7	-	wild-type, pH 8.5, 25°C	Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + HCO3-	-	Corynebacterium glutamicum	phosphate + oxaloacetate	-	?
phosphoenolpyruvate + HCO3-	-	Corynebacterium glutamicum DSM 20300	phosphate + oxaloacetate	-	?

Synonyms

Synonyms	Comment	Organism
NCgl1523	-	Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.005	-	phosphoenolpyruvate	mutant K653Q, pH 8.5, 25°C	Corynebacterium glutamicum
0.038	-	phosphoenolpyruvate	mutant K653R, pH 8.5, 25°C	Corynebacterium glutamicum
0.055	-	phosphoenolpyruvate	wild-type, pH 8.5, 25°C	Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0003	-	phosphoenolpyruvate	mutant K653Q, pH 8.5, 25°C	Corynebacterium glutamicum
0.0043	-	phosphoenolpyruvate	mutant K653R, pH 8.5, 25°C	Corynebacterium glutamicum
0.03	-	phosphoenolpyruvate	wild-type, pH 8.5, 25°C	Corynebacterium glutamicum

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Release 2023.1 (January 2023)