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Literature summary for 4.1.1.31 extracted from

  • Murmu, J.; Chinthapalli, B.; Raghavendra, A.S.
    Marked modulation by phosphate of phosphoenolpyruvate carboxylase in leaves of Amaranthus hypochondriacus, a NAD-ME type C4 plant: decrease in malate sensitivity but no change in the phosphorylation (2003), J. Exp. Bot., 54, 2661-2668.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
(S)-malate
-
 Amaranthus hypochondriacus
malate
-
 Amaranthus hypochondriacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 phosphoenolpyruvate pH 7.3, no addition of phosphate, enzyme from light-adapted leaves Amaranthus hypochondriacus
0.18
-
 phosphoenolpyruvate pH 7.3, addition of 30 mM phosphate, enzyme from light-adapted leaves Amaranthus hypochondriacus
0.33
-
 phosphoenolpyruvate pH 7.3, no addition of phosphate, enzyme from dark-adapted leaves Amaranthus hypochondriacus
0.49
-
 phosphoenolpyruvate pH 7.3, addition of 30 mM phosphate, enzyme from dark-adapted leaves Amaranthus hypochondriacus

Organism

Organism UniProt Comment Textmining
Amaranthus hypochondriacus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Amaranthus hypochondriacus

Source Tissue

Source Tissue Comment Organism Textmining
leaf dark-adapted and light adapted Amaranthus hypochondriacus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
 Amaranthus hypochondriacus phosphate + oxaloacetate
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.13
-
 (S)-malate pH 7.3, no addition of phosphate, enzyme from dark-adapted leaves Amaranthus hypochondriacus
0.21
-
 (S)-malate pH 7.3, no addition of phosphate, enzyme from light-adapted leaves Amaranthus hypochondriacus
0.34
-
 (S)-malate pH 7.3, addition of 30 mM phosohate, enzyme from dark-adapted leaves Amaranthus hypochondriacus
0.4
-
 (S)-malate pH 7.3, addition of 30 mM phosphate, enzyme from light-adapted leaves Amaranthus hypochondriacus