Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Polyethylene glycol | required for maximal activity | Kalanchoe fedtschenkoi |
General Stability | Organism |
---|---|
thiol protecting agents stabilize during purification | Kalanchoe fedtschenkoi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
malate | - |
Kalanchoe fedtschenkoi | |
phosphate | - |
Kalanchoe fedtschenkoi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
phosphoenolpyruvate | in absence of effectors, at 25°C | Kalanchoe fedtschenkoi | |
1.7 | - |
phosphoenolpyruvate | at 14°C | Kalanchoe fedtschenkoi | |
2.4 | - |
phosphoenolpyruvate | at 40°C | Kalanchoe fedtschenkoi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
4 * 105000, SDS-PAGE | Kalanchoe fedtschenkoi |
500000 | - |
gel filtration | Kalanchoe fedtschenkoi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Phosphoenolpyruvate + CO2 | Kalanchoe fedtschenkoi | possible role of the enzyme in controlling a circadian rhythm of CO2 fixation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kalanchoe fedtschenkoi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Kalanchoe fedtschenkoi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
- |
Kalanchoe fedtschenkoi |
Storage Stability | Organism |
---|---|
4°C, in (NH4)2SO4 suspension, 1 month stable | Kalanchoe fedtschenkoi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + CO2 | - |
Kalanchoe fedtschenkoi | phosphate + oxaloacetate | - |
? | |
Phosphoenolpyruvate + CO2 | possible role of the enzyme in controlling a circadian rhythm of CO2 fixation | Kalanchoe fedtschenkoi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 105000, SDS-PAGE | Kalanchoe fedtschenkoi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, only small activity loss below 50°C | Kalanchoe fedtschenkoi |
55 | 57 | 10 min, 50% denaturation | Kalanchoe fedtschenkoi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
double optimum at pH 5.8 and pH 7.8 | Kalanchoe fedtschenkoi |
7.8 | - |
double optimum at pH 5.8 and at pH 7.8 | Kalanchoe fedtschenkoi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7.8 | double optimum at pH 5.8 and at pH 7.8, minimum at pH 6.6 | Kalanchoe fedtschenkoi |