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Literature summary for 4.1.1.31 extracted from

  • Jones, R.; Wilkins, M.B.; Coggins, J.R.; Fewson, C.A.; Malcolm, A.D.B.
    Phosphoenolpyruvate carboxylase from the crassulacean plant Bryophyllum fedtschenkoi Hamet et Perrier (1978), Biochem. J., 175, 391-406.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Polyethylene glycol required for maximal activity Kalanchoe fedtschenkoi

General Stability

General Stability Organism
thiol protecting agents stabilize during purification Kalanchoe fedtschenkoi

Inhibitors

Inhibitors Comment Organism Structure
malate
-
Kalanchoe fedtschenkoi
phosphate
-
Kalanchoe fedtschenkoi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
phosphoenolpyruvate in absence of effectors, at 25°C Kalanchoe fedtschenkoi
1.7
-
phosphoenolpyruvate at 14°C Kalanchoe fedtschenkoi
2.4
-
phosphoenolpyruvate at 40°C Kalanchoe fedtschenkoi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
4 * 105000, SDS-PAGE Kalanchoe fedtschenkoi
500000
-
gel filtration Kalanchoe fedtschenkoi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Phosphoenolpyruvate + CO2 Kalanchoe fedtschenkoi possible role of the enzyme in controlling a circadian rhythm of CO2 fixation ?
-
?

Organism

Organism UniProt Comment Textmining
Kalanchoe fedtschenkoi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Kalanchoe fedtschenkoi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
-
Kalanchoe fedtschenkoi

Storage Stability

Storage Stability Organism
4°C, in (NH4)2SO4 suspension, 1 month stable Kalanchoe fedtschenkoi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
Kalanchoe fedtschenkoi phosphate + oxaloacetate
-
?
Phosphoenolpyruvate + CO2 possible role of the enzyme in controlling a circadian rhythm of CO2 fixation Kalanchoe fedtschenkoi ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 105000, SDS-PAGE Kalanchoe fedtschenkoi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, only small activity loss below 50°C Kalanchoe fedtschenkoi
55 57 10 min, 50% denaturation Kalanchoe fedtschenkoi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
double optimum at pH 5.8 and pH 7.8 Kalanchoe fedtschenkoi
7.8
-
double optimum at pH 5.8 and at pH 7.8 Kalanchoe fedtschenkoi

pH Range

pH Minimum pH Maximum Comment Organism
5.8 7.8 double optimum at pH 5.8 and at pH 7.8, minimum at pH 6.6 Kalanchoe fedtschenkoi