Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.30 extracted from

  • Brown, G.M.
    Pantothenylcysteine, a precursor of pantetheine in Lactobacillus helveticus (1957), J. Biol. Chem., 226, 651-661.
    View publication on PubMed
Search for more literature for 4.1.1.30

General Stability

General Stability Organism
dialysed at 4°C, pH 5.5-7.5, without loss in activity, cell extract Lactobacillus delbrueckii subsp. bulgaricus
dialysed at 4°C, pH 5.5-7.5, without loss in activity, cell extract Lactobacillus helveticus
MgCl2 and protamine inactivate Lactobacillus delbrueckii subsp. bulgaricus
MgCl2 and protamine inactivate Lactobacillus helveticus
no loss in activity after dialysis against Versene Lactobacillus delbrueckii subsp. bulgaricus
no loss in activity after dialysis against Versene Lactobacillus helveticus

Inhibitors

Inhibitors Comment Organism Structure
MgCl2 inactivates Lactobacillus delbrueckii subsp. bulgaricus
MgCl2 inactivates Lactobacillus helveticus
additional information
-
 Lactobacillus delbrueckii subsp. bulgaricus
additional information
-
 Lactobacillus helveticus
protamine inactivates Lactobacillus delbrueckii subsp. bulgaricus
protamine inactivates Lactobacillus helveticus

Localization

Localization Comment Organism GeneOntology No. Textmining
particle-bound
-
 Lactobacillus delbrueckii subsp. bulgaricus
-
-
particle-bound
-
 Lactobacillus helveticus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-[(R)-pantothenoyl]-L-cysteine Lactobacillus delbrueckii subsp. bulgaricus
-
 pantetheine + CO2
-
 ?
N-[(R)-pantothenoyl]-L-cysteine Lactobacillus helveticus
-
 pantetheine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Lactobacillus delbrueckii subsp. bulgaricus
-
-
-
Lactobacillus helveticus
-
-
-
no activity in Lactobacillus acidophilus
-
-
-
no activity in Lactobacillus arabinosus
-
-
-
no activity in Lactobacillus casei
-
-
-
no activity in Lactobacillus delbrueckii
-
-
-

Storage Stability

Storage Stability Organism
no loss in activity at 4°C or -20°C, pH 7.0, crude extract, 10-20 mg protein/ml Lactobacillus delbrueckii subsp. bulgaricus
no loss in activity at 4°C or -20°C, pH 7.0, crude extract, 10-20 mg protein/ml Lactobacillus helveticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-[(R)-pantothenoyl]-L-cysteine
-
 Lactobacillus delbrueckii subsp. bulgaricus pantetheine + CO2
-
 ?
N-[(R)-pantothenoyl]-L-cysteine
-
 Lactobacillus helveticus pantetheine + CO2
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
38
-
 soluble preparations stable up to 38°C for 3 h Lactobacillus delbrueckii subsp. bulgaricus
38
-
 soluble preparations stable up to 38°C for 3 h Lactobacillus helveticus
60
-
 rapid loss of activity after 30 min Lactobacillus delbrueckii subsp. bulgaricus
60
-
 rapid loss of activity after 30 min Lactobacillus helveticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.5 no sharply defined optimal value Lactobacillus delbrueckii subsp. bulgaricus
5.5 7.5 no sharply defined optimal value Lactobacillus helveticus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 rapid loss of activity below pH 5.5 and above pH 7.5, cell extract Lactobacillus delbrueckii subsp. bulgaricus
5.5 7.5 rapid loss of activity below pH 5.5 and above pH 7.5, cell extract Lactobacillus helveticus

Cofactor

Cofactor Comment Organism Structure
additional information no requirement Lactobacillus delbrueckii subsp. bulgaricus
additional information no requirement Lactobacillus helveticus