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Literature summary for 4.1.1.28 extracted from

  • Koyanagi, T.; Nakagawa, A.; Sakurama, H.; Yamamoto, K.; Sakurai, N.; Takagi, Y.; Minami, H.; Katayama, T.; Kumagai, H.
    Eukaryotic-type aromatic amino acid decarboxylase from the root colonizer Pseudomonas putida is highly specific for 3,4-dihydroxyphenyl-L-alanine, an allelochemical in the rhizosphere (2012), Microbiology, 158, 2965-2974.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.092
-
L-Dopa pH 8., 30°C Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88JU5
-
-
Pseudomonas putida KT 2240 Q88JU5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Dopa
-
Pseudomonas putida dopamine + CO2
-
?
L-Dopa
-
Pseudomonas putida KT 2240 dopamine + CO2
-
?
additional information enzyme is essentially inactive towards other aromatic amino acids such as 5-hydroxy-L-tryptophan, Lphenylalanine, L-tryptophan and L-tyrosine Pseudomonas putida ?
-
?
additional information enzyme is essentially inactive towards other aromatic amino acids such as 5-hydroxy-L-tryptophan, Lphenylalanine, L-tryptophan and L-tyrosine Pseudomonas putida KT 2240 ?
-
?
N-methyl-L-dopa
-
Pseudomonas putida N-methyldopamine + CO2 in addition, enzyme catalyses the decarboxylation-dependent oxidative deamination of alpha-methyl-dopa, but the oxidative reaction is considerably slower than the decarboxylation reaction ?
N-methyl-L-dopa
-
Pseudomonas putida KT 2240 N-methyldopamine + CO2 in addition, enzyme catalyses the decarboxylation-dependent oxidative deamination of alpha-methyl-dopa, but the oxidative reaction is considerably slower than the decarboxylation reaction ?

Synonyms

Synonyms Comment Organism
PP_2552
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
L-Dopa pH 8., 30°C Pseudomonas putida

Expression

Organism Comment Expression
Pseudomonas putida gene expression is induced by L-dopa up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
21
-
L-Dopa pH 8., 30°C Pseudomonas putida