Cloned (Comment) | Organism |
---|---|
expression n Escherichia coli | Levilactobacillus brevis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 9.2% residual activity | Levilactobacillus brevis | |
Fe2+ | 1 mM, 5.7% residual activity | Levilactobacillus brevis | |
Na2HPO4 | 1 mM, 67.2% residual activity | Levilactobacillus brevis | |
Ni2+ | 1 mM, 64.3% residual activity | Levilactobacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
L-tyrosine | pH 5.0, 40°C | Levilactobacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
2 * 70000, SDS-PAGE | Levilactobacillus brevis |
140000 | - |
gel filtration | Levilactobacillus brevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | J7GQ11 | - |
- |
Levilactobacillus brevis CGMCC 1.2028 | J7GQ11 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
133.5 | - |
pH 5.0, 40°C | Levilactobacillus brevis |
Storage Stability | Organism |
---|---|
4°C, 25 mM TrisHCl, pH 7.4, 150 mM NaCl, 4 weeks, over 80% of activity | Levilactobacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Dopa | 43.9% of the activity with L-tyrosine | Levilactobacillus brevis | dopamine + CO2 | - |
? | |
L-Dopa | 43.9% of the activity with L-tyrosine | Levilactobacillus brevis CGMCC 1.2028 | dopamine + CO2 | - |
? | |
L-Tyrosine | - |
Levilactobacillus brevis | Tyramine + CO2 | - |
? | |
L-Tyrosine | - |
Levilactobacillus brevis CGMCC 1.2028 | Tyramine + CO2 | - |
? | |
additional information | no substrates: L-phenylalanine, L-tryptophan | Levilactobacillus brevis | ? | - |
? | |
additional information | no substrates: L-phenylalanine, L-tryptophan | Levilactobacillus brevis CGMCC 1.2028 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 70000, SDS-PAGE | Levilactobacillus brevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Levilactobacillus brevis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
8.5% of maximum activity | Levilactobacillus brevis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
1 h, 14% residual activity | Levilactobacillus brevis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
343 | - |
L-tyrosine | pH 5.0, 40°C | Levilactobacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Levilactobacillus brevis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
inactive below | Levilactobacillus brevis |
5 | 8 | at least 95% of maximum activity | Levilactobacillus brevis |
9 | - |
inactive above | Levilactobacillus brevis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
7 days, 92% residual activity | Levilactobacillus brevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Levilactobacillus brevis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Levilactobacillus brevis | isoelectric focusing | - |
5.5 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
583 | - |
L-tyrosine | pH 5.0, 40°C | Levilactobacillus brevis |