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Literature summary for 4.1.1.25 extracted from

  • Torrens-Spence, M.P.; Liu, P.; Ding, H.; Harich, K.; Gillaspy, G.; Li, J.
    Biochemical evaluation of the decarboxylation and decarboxylation-deamination activities of plant aromatic amino acid decarboxylases (2013), J. Biol. Chem., 288, 2376-2387.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.25

Cloned(Commentary)

Cloned (Comment) Organism
-
 Arabidopsis thaliana
-
 Thalictrum flavum subsp. glaucum
-
 Petroselinum crispum

Protein Variants

Protein Variants Comment Organism
F338Y alteration in the primary activity from decarboxylation/deamination to decarboxylation. Mutant displays a very low activity to tyrosine, i.e. about 5% of its activity to phenylalanine, and strong activity to DOPA Arabidopsis thaliana
F346Y alteration in the primary activity from decarboxylation-deamination to decarboxylation. Mutant retains a small percentage of decarboxylation-deamination activity Petroselinum crispum
additional information generation of a chimeric protein composed of Thalictrum flavum tyrosine decarboxylase residues 1–300 and Petroselinum crispum romatic acetaldehyde synthase residues 300–514. The hybrid enzyme behaves primarily as a wild-type Petroselinum crispum acetaldehyde synthase Thalictrum flavum subsp. glaucum
additional information generation of a chimeric protein composed of Thalictrum flavum tyrosine decarboxylase residues 1–300 and Petroselinum crispum romatic acetaldehyde synthase residues 300–514. The hybrid enzyme behaves primarily as a wild-type Petroselinum crispum acetaldehyde synthase Petroselinum crispum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6
-
 L-phenylalanine mutant F338Y, pH 7., 25°C Arabidopsis thaliana
5.1
-
 L-phenylalanine wild-type, pH 7., 25°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8RY79
-
-
Petroselinum crispum Q06086
-
-
Thalictrum flavum subsp. glaucum Q9AXN7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + H2O
-
 Arabidopsis thaliana phenylethylamine + H2O2 reaction of mutant F338Y ?
L-tyrosine + H2O
-
 Petroselinum crispum 4-hydroxyphenylacetaldehyde + CO2 + NH3 enzyme catalyzes decarboxylation and subsequent deamination of substrate ?

Synonyms

Synonyms Comment Organism
TyrDC-2
-
 Petroselinum crispum

General Information

General Information Comment Organism
physiological function the phenylalanine residue in the catalytic loop region could serve as a signature residue to reliably distinguish plant arylalkylamine and aldehyde synthesizing aromaic amino acid decarboxylases Petroselinum crispum