Application | Comment | Organism |
---|---|---|
nutrition | information about enzyme may contribute to the prevention of tyramine formation during wine production and storage | Levilactobacillus brevis |
General Stability | Organism |
---|---|
complete inactivation of purified TDC by freezing at -20°C, unstable during purification, washing of purified extract with sodium acetate buffer, pH 5, by ultracentrifugation results in complete loss of activity, partially restored by 0.2 mM pyridoxal 5-phosphate | Levilactobacillus brevis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tyramine | mixed non-competitive inhibitor | Levilactobacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
L-tyrosine | pH 5, 25°C | Levilactobacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
2 * 70000, SDS-PAGE | Levilactobacillus brevis |
140000 | - |
gel filtration | Levilactobacillus brevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Tyrosine | Levilactobacillus brevis | tyrosine decarboxylation in lactobacilli | Tyramine + CO2 | - |
? | |
L-Tyrosine | Levilactobacillus brevis IOEB 9809 | tyrosine decarboxylation in lactobacilli | Tyramine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | - |
isolated from wine | - |
Levilactobacillus brevis IOEB 9809 | - |
isolated from wine | - |
Purification (Comment) | Organism |
---|---|
66fold | Levilactobacillus brevis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1058 | - |
pH 5, 25°C | Levilactobacillus brevis |
Storage Stability | Organism |
---|---|
4°, 0.2 mM pyridoxal 5-phosphate, 3 days, stable | Levilactobacillus brevis |
4°C, repeated storage, half-life: 1.5 days | Levilactobacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Tyrosine | highly specific for L-tyrosine | Levilactobacillus brevis | Tyramine + CO2 | - |
? | |
L-Tyrosine | tyrosine decarboxylation in lactobacilli | Levilactobacillus brevis | Tyramine + CO2 | - |
? | |
L-Tyrosine | highly specific for L-tyrosine | Levilactobacillus brevis IOEB 9809 | Tyramine + CO2 | - |
? | |
L-Tyrosine | tyrosine decarboxylation in lactobacilli | Levilactobacillus brevis IOEB 9809 | Tyramine + CO2 | - |
? | |
additional information | not: histidine, lysine, phenylalanine, tryptophan, ornithine, L-DOPA | Levilactobacillus brevis | ? | - |
? | |
additional information | not: histidine, lysine, phenylalanine, tryptophan, ornithine, L-DOPA | Levilactobacillus brevis IOEB 9809 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 70000, SDS-PAGE | Levilactobacillus brevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Levilactobacillus brevis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable up to for 10 min, after 20 min 75% loss of activity | Levilactobacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Levilactobacillus brevis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 9 | no activity below pH 2 or above pH 9 | Levilactobacillus brevis |
6 | - |
more than 90% of maximum activity at pH 6 | Levilactobacillus brevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | coenzyme dependence | Levilactobacillus brevis |