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Literature summary for 4.1.1.25 extracted from

  • Moreno-Arribas, V.; Lonvaud-Funel, A.
    Purification and characterization of tyrosine decarboxylase of Lactobacillus brevis IOEB 9809 isolated from wine (2001), FEMS Microbiol. Lett., 195, 103-107.
    View publication on PubMed

Application

Application Comment Organism
nutrition information about enzyme may contribute to the prevention of tyramine formation during wine production and storage Levilactobacillus brevis

General Stability

General Stability Organism
complete inactivation of purified TDC by freezing at -20°C, unstable during purification, washing of purified extract with sodium acetate buffer, pH 5, by ultracentrifugation results in complete loss of activity, partially restored by 0.2 mM pyridoxal 5’-phosphate Levilactobacillus brevis

Inhibitors

Inhibitors Comment Organism Structure
tyramine mixed non-competitive inhibitor Levilactobacillus brevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
L-tyrosine pH 5, 25°C Levilactobacillus brevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
2 * 70000, SDS-PAGE Levilactobacillus brevis
140000
-
gel filtration Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Tyrosine Levilactobacillus brevis tyrosine decarboxylation in lactobacilli Tyramine + CO2
-
?
L-Tyrosine Levilactobacillus brevis IOEB 9809 tyrosine decarboxylation in lactobacilli Tyramine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Levilactobacillus brevis
-
isolated from wine
-
Levilactobacillus brevis IOEB 9809
-
isolated from wine
-

Purification (Commentary)

Purification (Comment) Organism
66fold Levilactobacillus brevis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1058
-
pH 5, 25°C Levilactobacillus brevis

Storage Stability

Storage Stability Organism
4°, 0.2 mM pyridoxal 5’-phosphate, 3 days, stable Levilactobacillus brevis
4°C, repeated storage, half-life: 1.5 days Levilactobacillus brevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Tyrosine highly specific for L-tyrosine Levilactobacillus brevis Tyramine + CO2
-
?
L-Tyrosine tyrosine decarboxylation in lactobacilli Levilactobacillus brevis Tyramine + CO2
-
?
L-Tyrosine highly specific for L-tyrosine Levilactobacillus brevis IOEB 9809 Tyramine + CO2
-
?
L-Tyrosine tyrosine decarboxylation in lactobacilli Levilactobacillus brevis IOEB 9809 Tyramine + CO2
-
?
additional information not: histidine, lysine, phenylalanine, tryptophan, ornithine, L-DOPA Levilactobacillus brevis ?
-
?
additional information not: histidine, lysine, phenylalanine, tryptophan, ornithine, L-DOPA Levilactobacillus brevis IOEB 9809 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 70000, SDS-PAGE Levilactobacillus brevis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Levilactobacillus brevis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
stable up to for 10 min, after 20 min 75% loss of activity Levilactobacillus brevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Levilactobacillus brevis

pH Range

pH Minimum pH Maximum Comment Organism
2 9 no activity below pH 2 or above pH 9 Levilactobacillus brevis
6
-
more than 90% of maximum activity at pH 6 Levilactobacillus brevis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate coenzyme dependence Levilactobacillus brevis