Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.23 extracted from

  • Yuan, J.; Cardenas, A.M.; Gilbert, H.F.; Palzkill, T.
    Determination of the amino acid sequence requirements for catalysis by the highly proficient orotidine monophosphate decarboxylase (2011), Protein Sci., 20, 1891-1906.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D22G 30% of wild-type activity Escherichia coli
D71C 0.9% of wild-type activity Escherichia coli
D76C 1.1% of wild-type activity Escherichia coli
L130M 34% of wild-type activity Escherichia coli
Q201H 80% of wild-type activity Escherichia coli
T80S 100% of wild-type activity Escherichia coli
V167N 39% of wild-type activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
orotidine 5'-phosphate mutant T80S, pH 7.2, 25°C Escherichia coli
0.0012
-
orotidine 5'-phosphate mutant Q201H, pH 7.2, 25°C Escherichia coli
0.0028
-
orotidine 5'-phosphate wild-type, pH 7.2, 25°C Escherichia coli
0.0032
-
orotidine 5'-phosphate mutant D22G, pH 7.2, 25°C Escherichia coli
0.0041
-
orotidine 5'-phosphate mutant D76C, pH 7.2, 25°C Escherichia coli
0.005
-
orotidine 5'-phosphate mutant V167N, pH 7.2, 25°C Escherichia coli
0.0053
-
orotidine 5'-phosphate mutant L130M, pH 7.2, 25°C Escherichia coli
0.036
-
orotidine 5'-phosphate mutant D71C, pH 7.2, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Orotidine 5'-phosphate
-
Escherichia coli UMP + CO2
-
?

Synonyms

Synonyms Comment Organism
PyrF
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
orotidine 5'-phosphate mutant D71C, pH 7.2, 25°C Escherichia coli
0.7
-
orotidine 5'-phosphate mutant D76C, pH 7.2, 25°C Escherichia coli
3.2
-
orotidine 5'-phosphate mutant T80S, pH 7.2, 25°C Escherichia coli
13.7
-
orotidine 5'-phosphate mutant Q201H, pH 7.2, 25°C Escherichia coli
14.4
-
orotidine 5'-phosphate mutant D22G, pH 7.2, 25°C Escherichia coli
27
-
orotidine 5'-phosphate mutant L130M, pH 7.2, 25°C Escherichia coli
29.7
-
orotidine 5'-phosphate mutant V167N, pH 7.2, 25°C Escherichia coli
42
-
orotidine 5'-phosphate wild-type, pH 7.2, 25°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
14
-
orotidine 5'-phosphate mutant D71C, pH 7.2, 25°C Escherichia coli
170
-
orotidine 5'-phosphate mutant D76C, pH 7.2, 25°C Escherichia coli
4500
-
orotidine 5'-phosphate mutant D22G, pH 7.2, 25°C Escherichia coli
5100
-
orotidine 5'-phosphate mutant L130M, pH 7.2, 25°C Escherichia coli
5900
-
orotidine 5'-phosphate mutant V167N, pH 7.2, 25°C Escherichia coli
12000
-
orotidine 5'-phosphate mutant Q201H, pH 7.2, 25°C Escherichia coli
15000
-
orotidine 5'-phosphate mutant T80S, pH 7.2, 25°C Escherichia coli
15000
-
orotidine 5'-phosphate wild-type, pH 7.2, 25°C Escherichia coli