Cloned (Comment) | Organism |
---|---|
into the vector pET15b for expression in Escherichia coli BL21 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Homo sapiens | |
AMP | - |
Methanothermobacter thermautotrophicus | |
CMP | - |
Homo sapiens | |
CMP | - |
Methanothermobacter thermautotrophicus | |
dAMP | - |
Homo sapiens | |
dAMP | - |
Methanothermobacter thermautotrophicus | |
GMP | - |
Methanothermobacter thermautotrophicus | |
GMP | - |
Plasmodium falciparum | |
IMP | - |
Homo sapiens | |
IMP | - |
Methanothermobacter thermautotrophicus | |
IMP | - |
Plasmodium falciparum | |
TMP | - |
Homo sapiens | |
TMP | - |
Methanothermobacter thermautotrophicus | |
TMP | - |
Plasmodium falciparum | |
UMP | - |
Homo sapiens | |
UMP | - |
Methanothermobacter thermautotrophicus | |
UMP | - |
Plasmodium falciparum | |
XMP | - |
Homo sapiens | |
XMP | - |
Methanothermobacter thermautotrophicus | |
XMP | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
orotidine 5'-phosphate | - |
Plasmodium falciparum | |
0.0017 | - |
orotidine 5'-phosphate | - |
Homo sapiens | |
0.0048 | - |
orotidine 5'-phosphate | - |
Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27340 | - |
- |
Methanothermobacter thermautotrophicus |
31710 | - |
- |
Homo sapiens |
39850 | - |
- |
Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Homo sapiens | - |
UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | Plasmodium falciparum | - |
UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | Methanothermobacter thermautotrophicus | - |
UMP + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Methanothermobacter thermautotrophicus | O26232 | - |
- |
Plasmodium falciparum | Q8IJH3 | - |
- |
Purification (Comment) | Organism |
---|---|
using a Ni and a gel filtration column, the His6-tag is removed by thrombin digestion | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | - |
Homo sapiens | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | - |
Plasmodium falciparum | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | - |
Methanothermobacter thermautotrophicus | UMP + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ODCase | - |
Homo sapiens |
ODCase | - |
Plasmodium falciparum |
ODCase | - |
Methanothermobacter thermautotrophicus |
Orotidine monophosphate decarboxylase | - |
Homo sapiens |
Orotidine monophosphate decarboxylase | - |
Plasmodium falciparum |
Orotidine monophosphate decarboxylase | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Homo sapiens |
37 | - |
activity assay | Plasmodium falciparum |
55 | - |
activity assay | Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
orotidine 5'-phosphate | - |
Plasmodium falciparum | |
8.1 | - |
orotidine 5'-phosphate | - |
Homo sapiens | |
20.7 | - |
orotidine 5'-phosphate | - |
Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assay | Plasmodium falciparum |
7.5 | - |
activity assay | Homo sapiens |
7.5 | - |
activity assay | Methanothermobacter thermautotrophicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
XMP | - |
Homo sapiens | |
0.043 | - |
XMP | - |
Plasmodium falciparum | |
0.13 | - |
XMP | - |
Methanothermobacter thermautotrophicus | |
0.21 | - |
UMP | - |
Plasmodium falciparum | |
0.22 | - |
UMP | - |
Homo sapiens | |
0.33 | - |
UMP | - |
Methanothermobacter thermautotrophicus | |
1.2 | - |
CMP | - |
Methanothermobacter thermautotrophicus | |
1.4 | - |
CMP | - |
Homo sapiens | |
2.1 | - |
GMP | - |
Plasmodium falciparum | |
3.3 | - |
TMP | - |
Methanothermobacter thermautotrophicus | |
3.5 | - |
AMP | - |
Methanothermobacter thermautotrophicus | |
4 | - |
IMP | - |
Plasmodium falciparum | |
4.2 | - |
GMP | - |
Methanothermobacter thermautotrophicus | |
4.3 | - |
AMP | - |
Homo sapiens | |
5.7 | - |
IMP | - |
Methanothermobacter thermautotrophicus | |
7.2 | - |
IMP | - |
Homo sapiens | |
7.4 | - |
dAMP | - |
Homo sapiens | |
8.7 | - |
TMP | - |
Homo sapiens | |
10 | - |
TMP | - |
Plasmodium falciparum | |
10 | - |
dAMP | above 10 mM | Methanothermobacter thermautotrophicus |