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Literature summary for 4.1.1.23 extracted from

  • Poduch, E.; Wei, L.; Pai, E.F.; Kotra, L.P.
    Structural diversity and plasticity associated with nucleotides targeting orotidine monophosphate decarboxylase (2008), J. Med. Chem., 51, 432-438.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET15b for expression in Escherichia coli BL21 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
Homo sapiens
AMP
-
Methanothermobacter thermautotrophicus
CMP
-
Homo sapiens
CMP
-
Methanothermobacter thermautotrophicus
dAMP
-
Homo sapiens
dAMP
-
Methanothermobacter thermautotrophicus
GMP
-
Methanothermobacter thermautotrophicus
GMP
-
Plasmodium falciparum
IMP
-
Homo sapiens
IMP
-
Methanothermobacter thermautotrophicus
IMP
-
Plasmodium falciparum
TMP
-
Homo sapiens
TMP
-
Methanothermobacter thermautotrophicus
TMP
-
Plasmodium falciparum
UMP
-
Homo sapiens
UMP
-
Methanothermobacter thermautotrophicus
UMP
-
Plasmodium falciparum
XMP
-
Homo sapiens
XMP
-
Methanothermobacter thermautotrophicus
XMP
-
Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
orotidine 5'-phosphate
-
Plasmodium falciparum
0.0017
-
orotidine 5'-phosphate
-
Homo sapiens
0.0048
-
orotidine 5'-phosphate
-
Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27340
-
-
Methanothermobacter thermautotrophicus
31710
-
-
Homo sapiens
39850
-
-
Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Orotidine 5'-phosphate Homo sapiens
-
UMP + CO2
-
?
Orotidine 5'-phosphate Plasmodium falciparum
-
UMP + CO2
-
?
Orotidine 5'-phosphate Methanothermobacter thermautotrophicus
-
UMP + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Methanothermobacter thermautotrophicus O26232
-
-
Plasmodium falciparum Q8IJH3
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Ni and a gel filtration column, the His6-tag is removed by thrombin digestion Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Orotidine 5'-phosphate
-
Homo sapiens UMP + CO2
-
?
Orotidine 5'-phosphate
-
Plasmodium falciparum UMP + CO2
-
?
Orotidine 5'-phosphate
-
Methanothermobacter thermautotrophicus UMP + CO2
-
?

Synonyms

Synonyms Comment Organism
ODCase
-
Homo sapiens
ODCase
-
Plasmodium falciparum
ODCase
-
Methanothermobacter thermautotrophicus
Orotidine monophosphate decarboxylase
-
Homo sapiens
Orotidine monophosphate decarboxylase
-
Plasmodium falciparum
Orotidine monophosphate decarboxylase
-
Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
activity assay Homo sapiens
37
-
activity assay Plasmodium falciparum
55
-
activity assay Methanothermobacter thermautotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.7
-
orotidine 5'-phosphate
-
Plasmodium falciparum
8.1
-
orotidine 5'-phosphate
-
Homo sapiens
20.7
-
orotidine 5'-phosphate
-
Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
activity assay Plasmodium falciparum
7.5
-
activity assay Homo sapiens
7.5
-
activity assay Methanothermobacter thermautotrophicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0007
-
XMP
-
Homo sapiens
0.043
-
XMP
-
Plasmodium falciparum
0.13
-
XMP
-
Methanothermobacter thermautotrophicus
0.21
-
UMP
-
Plasmodium falciparum
0.22
-
UMP
-
Homo sapiens
0.33
-
UMP
-
Methanothermobacter thermautotrophicus
1.2
-
CMP
-
Methanothermobacter thermautotrophicus
1.4
-
CMP
-
Homo sapiens
2.1
-
GMP
-
Plasmodium falciparum
3.3
-
TMP
-
Methanothermobacter thermautotrophicus
3.5
-
AMP
-
Methanothermobacter thermautotrophicus
4
-
IMP
-
Plasmodium falciparum
4.2
-
GMP
-
Methanothermobacter thermautotrophicus
4.3
-
AMP
-
Homo sapiens
5.7
-
IMP
-
Methanothermobacter thermautotrophicus
7.2
-
IMP
-
Homo sapiens
7.4
-
dAMP
-
Homo sapiens
8.7
-
TMP
-
Homo sapiens
10
-
TMP
-
Plasmodium falciparum
10
-
dAMP above 10 mM Methanothermobacter thermautotrophicus