Cloned (Comment) | Organism |
---|---|
into the vector pET3d for expression in Escherichia coli cells | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
the structure of PfODCase is solved to a resolution of 1.5418 A | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-azauridine 5'-monophosphate | - |
Plasmodium falciparum | |
allopurinol-3-riboside 5'-monophosphate | - |
Plasmodium falciparum | |
pyrazofurin 5'-monophosphate | - |
Plasmodium falciparum | |
xanthosine 5'-monophosphate | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | - |
orotidine 5'-phosphate | - |
Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
subunit, determined by SDS-PAGE | Plasmodium falciparum |
76000 | - |
determined by gel filtration | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Plasmodium falciparum | - |
UMP + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8T6J6 | - |
- |
Purification (Comment) | Organism |
---|---|
using a Ni-NTA agarose column, the polyhistidine tag is removed with thrombin, the cleaved protein is further purified using a POROS-HS cation exchange and a Superdex-200 size-exclusion column | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | - |
Plasmodium falciparum | UMP + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
Orotidine 5'-monophosphate decarboxylase | - |
Plasmodium falciparum |
PfODCase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
activity assay | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000036 | - |
pyrazofurin 5'-monophosphate | - |
Plasmodium falciparum | |
0.0000044 | - |
xanthosine 5'-monophosphate | - |
Plasmodium falciparum | |
0.000012 | - |
6-azauridine 5'-monophosphate | - |
Plasmodium falciparum | |
0.00024 | - |
allopurinol-3-riboside 5'-monophosphate | - |
Plasmodium falciparum |