Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-iodouridine 5'-monophosphate | i.e. 6-iodo-UMP, irreversible inhibition, mass spectral analysis of the enzyme-inhibitor complex, binding to the enzyme is accompanied by loss of two protons and the iodo moiety, covalent bond formation | Methanothermobacter thermautotrophicus | |
6-iodouridine 5'-monophosphate | i.e. 6-iodo-UMP, irreversible inhibition, mass spectral analysis of the enzyme-inhibitor complex, binding to the enzyme is accompanied by loss of two protons and the iodo moiety, covalent bond formation, the inhibitor exhibits potent antiplasmodial activity | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Methanothermobacter thermautotrophicus | - |
UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | Plasmodium falciparum | - |
UMP + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Plasmodium falciparum | Q8IJH3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | - |
Methanothermobacter thermautotrophicus | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | - |
Plasmodium falciparum | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | the enzyme catalyzes the decarboxylation of orotidine 5'-monophosphate without any covalent intermediates, active site residues in ODCase are involved in an extensive hydrogen-bonding network, active site Lys42 | Methanothermobacter thermautotrophicus | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | the enzyme catalyzes the decarboxylation of orotidine 5'-monophosphate without any covalent intermediates, active site residues in ODCase are involved in an extensive hydrogen-bonding network, active site Lys42 | Plasmodium falciparum | UMP + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ODCase | - |
Methanothermobacter thermautotrophicus |
ODCase | - |
Plasmodium falciparum |
orotidine 5-monophosphate decarboxylase | - |
Methanothermobacter thermautotrophicus |
orotidine 5-monophosphate decarboxylase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Methanothermobacter thermautotrophicus |
22 | - |
assay at room temperature | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanothermobacter thermautotrophicus |
7.5 | - |
assay at | Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0044 | 0.0062 | pH 7.5, 22°C, with different Plasmodium falciparum isolates | Plasmodium falciparum | 6-iodo-UMP |