Cloned (Comment) | Organism |
---|---|
functional enzyme expression in Escherichia coli | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, seeding method in a hanging drop, 20°C, 10 mg/ml protein in in 50 mM Tris-HCl, pH 8.0, containing 300 mM NaCl and 5 mM dithiothreitol, method optimization, X-ray diffraction structure determination and analysis at 2.7 A resolution | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, active recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
76000 | - |
active recombinant enzyme, gel filtration | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Plasmodium falciparum | final step in the de novo synthesis of uridine 5'-monophosphate, UMP, defects in the enzyme are lethal in the malaria parasite | UMP + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
functional recombinant enzyme 300fold from Escherichia coli by nickel affinity chromatography and gel filtration | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | - |
purified recombinant enzyme | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | - |
Plasmodium falciparum | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | final step in the de novo synthesis of uridine 5'-monophosphate, UMP, defects in the enzyme are lethal in the malaria parasite | Plasmodium falciparum | UMP + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, active recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
OMP decarboxylase | - |
Plasmodium falciparum |
OMPDC | - |
Plasmodium falciparum |
orotidine 5-monophosphate decarboxylase | - |
Plasmodium falciparum |