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Literature summary for 4.1.1.23 extracted from
- Extremely thermostable orotidine-5'-monophosphate decarboxylase from the archaeon Sulfolobus acidocaldarius (1999), FEMS Microbiol. Lett., 174, 81-87.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | 0.1 mM, 2.2fold stimulation | Sulfolobus acidocaldarius |  |
| 5,5'-dithiobis(2-nitrobenzoic acid) | 0.1 mM, at 70°C, 2.2fold activation | Sulfolobus acidocaldarius | |
| 6-Azauracil | 1 mM, 1.2fold activation | Sulfolobus acidocaldarius | |
| N-ethylmaleimide | 10 mM, at 70°C, 17.8fold activation | Sulfolobus acidocaldarius | |
| N-ethylmaleimide | 10 mM, 18fold stimulation | Sulfolobus acidocaldarius | |
| Uracil | 1 mM, 1.3fold stimulation | Sulfolobus acidocaldarius | |
General Stability
| General Stability | Organism |
|---|---|
| orotidine 5'-phosphate and UMP stabilize | Sulfolobus acidocaldarius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-fluorouracil | 1 mM, 14% inhibition | Sulfolobus acidocaldarius | |
| Dimethylsulfoxide | 20% v/v, 8% inhibition | Sulfolobus acidocaldarius | |
| EDTA | 10 mM, 6% inhibition; 10 mM, at 70°C, weak inhibition | Sulfolobus acidocaldarius | |
| Guanidine-HCl | 2.0 M, complete inhibition | Sulfolobus acidocaldarius | |
| Guanidinium chloride | 1 M, at 70°C, 81.4% inhibition | Sulfolobus acidocaldarius | |
| phosphate | low activity in phosphate buffers, inhibitory or destabilizing effect | Sulfolobus acidocaldarius | |
| SDS | 1%, at 70°C, complete inactivation; 1%, complete inhibition | Sulfolobus acidocaldarius | |
| UMP | 1 mM, 19% inhibition; 1 mM, at 70°C, 19% inhibition | Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
2 * 22000, SDS-PAGE | Sulfolobus acidocaldarius |
| 46000 | - |
gel filtration | Sulfolobus acidocaldarius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Orotidine 5'-phosphate | Sulfolobus acidocaldarius | - |
UMP + CO2 | - |
? | |
| Orotidine 5'-phosphate | Sulfolobus acidocaldarius DG6 / ATCC 49426 | - |
UMP + CO2 | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| guanidine-HCl | at 70°C, the enzyme is inactivated by guanidinium chloride at 1 M or higher concentrations | Sulfolobus acidocaldarius |
| SDS | at 70°C, the enzyme is inactivated by 1% SDS or higher concentrations | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | - |
- |
- |
| Sulfolobus acidocaldarius DG6 / ATCC 49426 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
| 1420fold | Sulfolobus acidocaldarius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0124 | - |
pH 8.0, 70°C | Sulfolobus acidocaldarius |
| 12.4 | - |
pH 8.5, 70°C | Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Orotidine 5'-phosphate | - |
Sulfolobus acidocaldarius | UMP + CO2 | - |
? | |
| Orotidine 5'-phosphate | - |
Sulfolobus acidocaldarius DG6 / ATCC 49426 | UMP + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 22000, SDS-PAGE | Sulfolobus acidocaldarius |
| homodimer | 2 * 22000, SDS-PAGE | Sulfolobus acidocaldarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Orotidine-5'-monophosphate decarboxylase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Sulfolobus acidocaldarius |
| 98 | - |
- |
Sulfolobus acidocaldarius |
| 98 | - |
above | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable | Sulfolobus acidocaldarius |
| 85 | - |
or above, extremely thermostable in presence of substrate or product, considerable thermal inactivation in their absence | Sulfolobus acidocaldarius |
| 85 | - |
in the absence of substrate and product, however, a considerable thermal inactivation was observed at 85°C or above | Sulfolobus acidocaldarius |
| 90 | - |
10 min, 90% loss of activity | Sulfolobus acidocaldarius |
| 90 | - |
10 min, over 90% loss of activity, in absence of substrate and product | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
broad pH-optimum centered about pH 8 | Sulfolobus acidocaldarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Sulfolobus acidocaldarius |
| 8.5 | - |
assay at | Sulfolobus acidocaldarius |
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