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Literature summary for 4.1.1.22 extracted from

  • Furuta, K.; Nakayama, K.; Sugimoto, Y.; Ichikawa, A.; Tanaka, S.
    Activation of histidine decarboxylase through post-translational cleavage by caspase-9 in a mouse mastocytoma P-815 (2007), J. Biol. Chem., 282, 13438-13446.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Butyrate highly activating for the wild-type enzyme Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of 5'-3fold FLAG-tagged and 3'-HA-tagged enzyme in 293FT and P-815 cells using a lentiviral expression system Mus musculus

Protein Variants

Protein Variants Comment Organism
C552A site-directed mutagenesis, the mutant shows similar cleavage by caspase-9 as the wild-type enzyme Mus musculus
D517A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
D518A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
D518A/D550A/D551A site-directed mutagenesis, the mutant shows highly reduced activation by butyrate compared to the wild-type enzyme Mus musculus
D547A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
D547A/P548A/F549A site-directed mutagenesis, the mutant shows highly reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
D550A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
D550A/D551A site-directed mutagenesis, the mutant shows highly reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
D550A/D551A/C552A site-directed mutagenesis, the mutant shows highly reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
D551A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
F549A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
I525A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
K524A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
K527A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
additional information construction of deletion mutants with altered activation by butyrate and cleavage by caspase-9, overview Mus musculus
P519A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
P548A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
Q521A site-directed mutagenesis, the mutant shows similar cleavage by caspase-9 as the wild-type enzyme Mus musculus
R523A site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus
T544A/M545A/P546A site-directed mutagenesis, the mutant shows highly reduced cleavage by caspase-9 compared to the wild-type enzyme Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
2 * 53000, SDS-PAGE, 2 * 74000, recombinant 5-3fold FLAG-tagged and 3-HA-tagged enzyme, SDS-PAGE Mus musculus
74000
-
2 * 53000, SDS-PAGE, 2 * 74000, recombinant 5-3fold FLAG-tagged and 3-HA-tagged enzyme, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-histidine Mus musculus HDC is the rate-limiting enzyme for histamine synthesis histamine + CO2
-
?
L-histidine Mus musculus BDF1 HDC is the rate-limiting enzyme for histamine synthesis histamine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
BDF1 mice
-
Mus musculus BDF1
-
BDF1 mice
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification post-translational cleavage by caspase-9 in a mouse mastocytoma P-815, residues D547-D551 and D517-K527 are important, overview Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
mastocytoma cell
-
Mus musculus
-
P-815 cell peritoneal cavity Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-histidine
-
Mus musculus histamine + CO2
-
?
L-histidine HDC is the rate-limiting enzyme for histamine synthesis Mus musculus histamine + CO2
-
?
L-histidine
-
Mus musculus BDF1 histamine + CO2
-
?
L-histidine HDC is the rate-limiting enzyme for histamine synthesis Mus musculus BDF1 histamine + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE, 2 * 74000, recombinant 5'-3fold FLAG-tagged and 3'-HA-tagged enzyme, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
HDC
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Mus musculus