Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Butyrate | highly activating for the wild-type enzyme | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of 5'-3fold FLAG-tagged and 3'-HA-tagged enzyme in 293FT and P-815 cells using a lentiviral expression system | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C552A | site-directed mutagenesis, the mutant shows similar cleavage by caspase-9 as the wild-type enzyme | Mus musculus |
D517A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
D518A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
D518A/D550A/D551A | site-directed mutagenesis, the mutant shows highly reduced activation by butyrate compared to the wild-type enzyme | Mus musculus |
D547A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
D547A/P548A/F549A | site-directed mutagenesis, the mutant shows highly reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
D550A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
D550A/D551A | site-directed mutagenesis, the mutant shows highly reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
D550A/D551A/C552A | site-directed mutagenesis, the mutant shows highly reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
D551A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
F549A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
I525A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
K524A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
K527A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
additional information | construction of deletion mutants with altered activation by butyrate and cleavage by caspase-9, overview | Mus musculus |
P519A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
P548A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
Q521A | site-directed mutagenesis, the mutant shows similar cleavage by caspase-9 as the wild-type enzyme | Mus musculus |
R523A | site-directed mutagenesis, the mutant shows reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
T544A/M545A/P546A | site-directed mutagenesis, the mutant shows highly reduced cleavage by caspase-9 compared to the wild-type enzyme | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE, 2 * 74000, recombinant 5-3fold FLAG-tagged and 3-HA-tagged enzyme, SDS-PAGE | Mus musculus |
74000 | - |
2 * 53000, SDS-PAGE, 2 * 74000, recombinant 5-3fold FLAG-tagged and 3-HA-tagged enzyme, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-histidine | Mus musculus | HDC is the rate-limiting enzyme for histamine synthesis | histamine + CO2 | - |
? | |
L-histidine | Mus musculus BDF1 | HDC is the rate-limiting enzyme for histamine synthesis | histamine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
BDF1 mice | - |
Mus musculus BDF1 | - |
BDF1 mice | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | post-translational cleavage by caspase-9 in a mouse mastocytoma P-815, residues D547-D551 and D517-K527 are important, overview | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mastocytoma cell | - |
Mus musculus | - |
P-815 cell | peritoneal cavity | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-histidine | - |
Mus musculus | histamine + CO2 | - |
? | |
L-histidine | HDC is the rate-limiting enzyme for histamine synthesis | Mus musculus | histamine + CO2 | - |
? | |
L-histidine | - |
Mus musculus BDF1 | histamine + CO2 | - |
? | |
L-histidine | HDC is the rate-limiting enzyme for histamine synthesis | Mus musculus BDF1 | histamine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE, 2 * 74000, recombinant 5'-3fold FLAG-tagged and 3'-HA-tagged enzyme, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
HDC | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mus musculus |