Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Morganella morganii |
Protein Variants | Comment | Organism |
---|---|---|
H231F | mutant enzymes His231Phe and His231Arg are inactive | Morganella morganii |
H231N | mutant enzyme His231Asn is 0.2% as active as the wild-type enzyme | Morganella morganii |
H231Q | mutant His231Gln is 12% as active as the wild-type enzyme | Morganella morganii |
H231R | mutant enzymes His231Phe and His231Arg are inactive | Morganella morganii |
K232A | mutant enzyme Lys232Ala is inactive but retains ability to bind both pyridoxal 5'-phosphate and His efficiently | Morganella morganii |
additional information | none of the four residues Met233, Cys230, Cys239 and Ser322 are essential for activity, altough all replacements reduce the activity of the enzyme significantly | Morganella morganii |
S229A | mutant Ser229Ala or Ser229Cys are about 7% as active as the wild-type enzyme | Morganella morganii |
S229C | mutant Ser229Ala or Ser229Cys are about 7% as active as the wild-type enzyme | Morganella morganii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Morganella morganii | - |
- |
- |
Morganella morganii AM-15 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Morganella morganii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-His | - |
Morganella morganii | Histamine + CO2 | - |
? | |
L-His | - |
Morganella morganii AM-15 | Histamine + CO2 | - |
? |