Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Hydroxy-5-nitrobenzylbromide | - |
Rattus norvegicus | |
Co2+ | 0.1 mM, 20% decrease of activity | Rattus norvegicus | |
Cu2+ | 0.1 mM, strong inhibition | Rattus norvegicus | |
Cys | - |
Rattus norvegicus | |
dithiothreitol | - |
Rattus norvegicus | |
Hg2+ | 0.1 mM, strong inhibition | Rattus norvegicus | |
histamine | at high concentrations | Rattus norvegicus | |
NEM | - |
Rattus norvegicus | |
Ni2+ | 0.1 mM, 15% decrease of activity | Rattus norvegicus | |
p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
pyridoxal 5'-phosphate | non-competitive inhibition with respect to His, at high concentrations | Rattus norvegicus | |
reduced glutathione | - |
Rattus norvegicus | |
Semicarbazide | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 0.1 mM, enhances activity by 7-8% | Rattus norvegicus | |
Mn2+ | 0.1 mM, enhances activity by 7-8% | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gastric mucosa | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-His | strictly specific for L-His | Rattus norvegicus | Histamine + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
- |
Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | 60 | 47°C: about 50% of maximal activity, 60°C: about 75% of maximal activity, no activity detectable at 65°C | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable for at least 30 min, without pyridoxal 5'-phosphate | Rattus norvegicus |
50 | - |
40% loss of activity after 30 min, 5fold increase of half-life in presence of saturating concentrations of pyridoxal 5'-phosphate | Rattus norvegicus |
55 | - |
50% loss of activity in 13 min, 5fold increase of half-life in presence of saturating concentrations of pyridoxal 5'-phosphate | Rattus norvegicus |
57 | - |
50% loss of activity in 4 min, 5fold increase of half-life in presence of saturating concentrations of pyridoxal 5'-phosphate | Rattus norvegicus |
60 | - |
50% loss of activity in 2 min, 5fold increase of half-life in presence of saturating concentrations of pyridoxal 5'-phosphate | Rattus norvegicus |
64 | - |
50% loss of activity in 30 s, 5fold increase of half-life in presence of saturating concentrations of pyridoxal 5'-phosphate | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required | Rattus norvegicus | |
pyridoxal 5'-phosphate | tightly bound to the apoenzyme. Non-competitive inhibition at high concentrations | Rattus norvegicus |