General Stability | Organism |
---|---|
under the condition of enzyme/support ratio at 1:20, pH 9, with 1.5 M (NH4)2SO4, 22°C, and shaking at 30 rpm for 24 h, activity recovery of Eupergit C-immobilized enzyme reaches 90% | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.46 | - |
oxalate | soluble enzyme, citrate buffer (0.05 M, pH 4.0), at 37°C | Bacillus subtilis | |
22.61 | - |
oxalate | Eupergit C-immobilized enzyme, citrate buffer (0.05 M, pH 4.0), at 37°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | Mn2+ binding to the enzyme is not easy to release | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalate + H+ | - |
Bacillus subtilis | formate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OXDC | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
soluble and immobilized enzyme | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | the soluble enzyme shows at least more than 50% activity between 30 and 70°C. The immobilized enzyme almost retains its full maximum activity at 70°C (7% decrease of activity), while the soluble enzyme shows about 50% activity at 70°C | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | both soluble and immobilized enzyme lose their activity continuously at 60°C treatment (1 h at pH 4.0). After thermal treatment for 10 min, 46.07% activity remains for the immobilized enzyme an keep 13.86% activity after 1 h of thermal treatment, while the soluble one loses its activity completely | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
soluble and immobilized enzyme | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | soluble and immobilized enzyme. When decreasing pH to 3.0, the soluble enzyme loses 76% of its original activity, but there is only 41% loss for the immobilized enzyme | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
both the soluble and immobilized enzyme are stable after 6 h of treatment with buffer at a pH higher than 4.5, and no obvious activity decrease are observed. There is 36.9% activity preserved in the immobilized enzyme after 6 h of treatment with a pH 3.5 buffer, and 4.2% activity remains in the soluble enzyme | Bacillus subtilis |