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Literature summary for 4.1.1.2 extracted from

  • Lin, R.; Wu, R.; Huang, X.; Xie, T.
    Immobilization of oxalate decarboxylase to Eupergit and properties of the immobilized enzyme (2011), Prep. Biochem. Biotechnol., 41, 154-165.
    View publication on PubMed
Search for more literature for 4.1.1.2

General Stability

General Stability Organism
under the condition of enzyme/support ratio at 1:20, pH 9, with 1.5 M (NH4)2SO4, 22°C, and shaking at 30 rpm for 24 h, activity recovery of Eupergit C-immobilized enzyme reaches 90% Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15.46
-
 oxalate soluble enzyme, citrate buffer (0.05 M, pH 4.0), at 37°C Bacillus subtilis
22.61
-
 oxalate Eupergit C-immobilized enzyme, citrate buffer (0.05 M, pH 4.0), at 37°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ Mn2+ binding to the enzyme is not easy to release Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxalate + H+
-
 Bacillus subtilis formate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
OXDC
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 soluble and immobilized enzyme Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 the soluble enzyme shows at least more than 50% activity between 30 and 70°C. The immobilized enzyme almost retains its full maximum activity at 70°C (7% decrease of activity), while the soluble enzyme shows about 50% activity at 70°C Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 both soluble and immobilized enzyme lose their activity continuously at 60°C treatment (1 h at pH 4.0). After thermal treatment for 10 min, 46.07% activity remains for the immobilized enzyme an keep 13.86% activity after 1 h of thermal treatment, while the soluble one loses its activity completely Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 soluble and immobilized enzyme Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
3 5 soluble and immobilized enzyme. When decreasing pH to 3.0, the soluble enzyme loses 76% of its original activity, but there is only 41% loss for the immobilized enzyme Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 both the soluble and immobilized enzyme are stable after 6 h of treatment with buffer at a pH higher than 4.5, and no obvious activity decrease are observed. There is 36.9% activity preserved in the immobilized enzyme after 6 h of treatment with a pH 3.5 buffer, and 4.2% activity remains in the soluble enzyme Bacillus subtilis