Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | pH-dependent induction in acidic growth media, particularly at pH 5, but not by oxalate | Bacillus subtilis | |
O2 | dioxygen-dependent reaction suggesting the role of a transition metal in catalysis | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bacillus subtilis | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | presence of a metal ion, role of a transition metal in catalysis | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43407 | - |
5 * 44000, SDS-PAGE, 5 * 43407, calculated from the amino acid sequence, pentamer, but it is possible, that YvrK is actually a hexamer | Bacillus subtilis |
44000 | - |
5 * 44000, SDS-PAGE, 5 * 43407, calculated from the amino acid sequence, pentamer, but it is possible, that YvrK is actually a hexamer | Bacillus subtilis |
220000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalate + H+ | Bacillus subtilis | oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation | formate + CO2 | - |
? | |
oxalate + H+ | Bacillus subtilis 168 | oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation | formate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis 168 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1000fold | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
-20°C, frozen in liquid nitrogen, pH buffered at or near 7, stable | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalate + H+ | acts exclusively on oxalate | Bacillus subtilis | formate + CO2 | - |
? | |
oxalate + H+ | oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation | Bacillus subtilis | formate + CO2 | - |
? | |
oxalate + H+ | acts exclusively on oxalate | Bacillus subtilis 168 | formate + CO2 | - |
? | |
oxalate + H+ | oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation | Bacillus subtilis 168 | formate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | 5 * 44000, SDS-PAGE, 5 * 43407, calculated from the amino acid sequence, pentamer, but it is possible, that YvrK is actually a hexamer | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
More | belongs to the cupin superfamily, bicupin | Bacillus subtilis |
YvrK | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7.5 | 70% of maximum activity at pH 3, no activity at pH 7.5 | Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | predicted value | - |
5.1 |
Bacillus subtilis | determined using PhastSystem | - |
6.1 |