Cloned (Comment) | Organism |
---|---|
- |
Flammulina velutipes |
General Stability | Organism |
---|---|
retains 45% of its activity after incubation with 10% SDS for 30 min at room temperature. Almost complete loss of activity after 30 min, in presence of 10% SDS | Flammulina velutipes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | competitive | Flammulina velutipes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
oxalate | - |
Flammulina velutipes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, enzyme decglycosylated by endo-beta-N-acetylglucosaminidase, SDS-PAGE | Flammulina velutipes |
64000 | - |
x * 64000, glycosylated enzyme, SDS-PAGE | Flammulina velutipes |
560000 | - |
the high apparent MW obtained by gel filtration can be due to the tendency of certain glycoproteins to interact noncovalently in solution, gel filtration | Flammulina velutipes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Oxalate | Flammulina velutipes | induction by oxalate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flammulina velutipes | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 15% neutral sugar content | Flammulina velutipes |
Purification (Comment) | Organism |
---|---|
- |
Flammulina velutipes |
Storage Stability | Organism |
---|---|
4°C, 1 mg/ml protein, 0.02 M potassium acetate, more than 70% of the initial activity is retained after 4 months | Flammulina velutipes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Oxalate | - |
Flammulina velutipes | Formate + CO2 | - |
? | |
Oxalate | induction by oxalate | Flammulina velutipes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, enzyme decglycosylated by endo-beta-N-acetylglucosaminidase, SDS-PAGE | Flammulina velutipes |
? | x * 64000, glycosylated enzyme, SDS-PAGE | Flammulina velutipes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
30 min, in presence of 10% SDS, retains 45% of its activity | Flammulina velutipes |
60 | - |
30 min, in presence of 10% SDS, complete inactivation | Flammulina velutipes |
80 | - |
20 min, 22% loss of activity | Flammulina velutipes |
96 | - |
10 min, complete inactivation | Flammulina velutipes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
- |
Flammulina velutipes |